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  4. Vast Differences in Strain-Level Diversity in the Gut Microbiota of Two Closely Related Honey Bee Species.
 
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Titre

Vast Differences in Strain-Level Diversity in the Gut Microbiota of Two Closely Related Honey Bee Species.

Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Current Biology  
Auteur(s)
Ellegaard, K.M.
Auteure/Auteur
Suenami, S.
Auteure/Auteur
Miyazaki, R.
Auteure/Auteur
Engel, P.
Auteure/Auteur
Liens vers les personnes
Engel, Philipp  
Ellegaard, Kirsten  
Liens vers les unités
Dép. microbiologie fondamentale  
ISSN
1879-0445
Statut éditorial
Publié
Date de publication
2020-07-06
Volume
30
Numéro
13
Première page
2520
Dernière page/numéro d’article
2531.e7
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Most bacterial species encompass strains with vastly different gene content. Strain diversity in microbial communities is therefore considered to be of functional importance. Yet little is known about the extent to which related microbial communities differ in diversity at this level and which underlying mechanisms may constrain and maintain strain-level diversity. Here, we used shotgun metagenomics to characterize and compare the gut microbiota of two honey bee species, Apis mellifera and Apis cerana, which diverged about 6 mya. Although the host species are colonized largely by the same bacterial 16S rRNA phylotypes, we find that their communities are host specific when analyzed with genomic resolution. Moreover, despite their similar ecology, A. mellifera displayed a much higher diversity of strains and functional gene content in the microbiota compared to A. cerana, both per colony and per individual bee. In particular, the gene repertoire for polysaccharide degradation was massively expanded in the microbiota of A. mellifera relative to A. cerana. Bee management practices, divergent ecological adaptation, or habitat size may have contributed to the observed differences in microbiota genomic diversity of these key pollinator species. Our results illustrate that the gut microbiota of closely related animal hosts can differ vastly in genomic diversity while displaying similar levels of diversity based on the 16S rRNA gene. Such differences are likely to have consequences for gut microbiota functioning and host-symbiont interactions, highlighting the need for metagenomic studies to understand the ecology and evolution of microbial communities.
Sujets

Animals

Bacteria/classificati...

Bacteria/genetics

Bacteria/isolation & ...

Bees/microbiology

Gastrointestinal Micr...

RNA, Bacterial/analys...

RNA, Ribosomal, 16S/a...

Species Specificity

Apis cerana

Apis mellifera

gut microbiota

honey bee

host associated

metagenomics

pan-genome

species

strain-level diversit...

PID Serval
serval:BIB_C8719CEC381F
DOI
10.1016/j.cub.2020.04.070
PMID
32531278
WOS
000548514700009
Permalien
https://iris.unil.ch/handle/iris/185516
Open Access
Oui
Date de création
2020-06-25T14:00:15.234Z
Date de création dans IRIS
2025-05-21T01:22:25Z
Fichier(s)
En cours de chargement...
Vignette d'image
Nom

Vast Differences in Strain-Level Diversity in the Gut Microbiota of Two Closely Related Honey Bee Species.pdf

Version du manuscrit

published

Licence

https://creativecommons.org/licenses/by-nc-nd/4.0

Taille

8.08 MB

Format

Adobe PDF

PID Serval

serval:BIB_C8719CEC381F.P001

URN

urn:nbn:ch:serval-BIB_C8719CEC381F0

Somme de contrôle

(MD5):9cc7fef913185dc4b53eb335ea5cf25e

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