Titre
Remaining rod activity mediates visual behavior in adult Rpe65-/- mice.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Cachafeiro, M.
Auteure/Auteur
Bemelmans, A.P.
Auteure/Auteur
Canola, K.
Auteure/Auteur
Pignat, V.
Auteure/Auteur
Crippa, S.V.
Auteure/Auteur
Kostic, C.
Auteure/Auteur
Arsenijevic, Y.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1552-5783
Statut éditorial
Publié
Date de publication
2010
Volume
51
Numéro
12
Première page
6835
Dernière page/numéro d’article
6842
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Résumé
Purpose: C57/Bl6, Cpfl1-/- (Cone photoreceptors function loss 1; pure rod function), Gnat1alpha-/- (rod alpha-transducin; pure cone function) and Rpe65-/-;Rho-/- double knock-out mice were studied in order to distinguish the respective contributions of the different photoreceptor (PR) systems that enable light perception and mediate a visual reflex in adult Rpe65-/- mice using a simple behavioural procedure. Methods: Visual function was estimated using a rotating automatized optomotor drum covered with vertical black and white stripes at spatial frequencies of 0.025 to 0.5 cycles per degree (cpd) in both photopic and scotopic conditions. To evaluate the contribution as well as the light intensity threshold of each PR system, we tested the mouse strains with different luminances. Results: Stripe rotation elicits head movements in wild-type (WT) animals in photopic and scotopic conditions depending on the spatial frequency, whereas Cpfl1-/- mice show a reduced activity in the photopic condition and Gnat1alpha-/- mice an almost absent response in the scotopic condition. Interestingly, a robust visual response is obtained with Rpe65-/- knockout mice at 0.075 cpd and 0.1 cpd in the photopic condition. The residual rod function in the Rpe65-/- animals was demonstrated by testing Rpe65-/-;Rho-/- mice that present no response in photopic conditions. Conclusions: The optomotor test is a simple method to estimate the visual function, and to evaluate the respective contributions of rod and cone systems. Using this test, we demonstrate that in Rpe65-/- mice, devoid of functional cones and of detectable 11-cis-retinal protein, rods mimic in part the cone function by mediating vision in photopic conditions.
Sujets
PID Serval
serval:BIB_DE0563D3C273
PMID
Open Access
Oui
Date de création
2010-10-13T14:18:05.193Z
Date de création dans IRIS
2025-05-20T22:50:16Z
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Nom
z7g01210006835.pdf
Version du manuscrit
published
Taille
1.08 MB
Format
Adobe PDF
PID Serval
serval:BIB_DE0563D3C273.P001
URN
urn:nbn:ch:serval-BIB_DE0563D3C2737
Somme de contrôle
(MD5):aad03adf43eb99b5fe97e427c79fc1be