Titre
Novel use of a whole cell E. coli bioreporter as a urinary exposure biomarker.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Environmental Science and Technology
Auteur(s)
Ewis, C.L.
Auteure/Auteur
Beggah, S.
Auteure/Auteur
Pook, C.
Auteure/Auteur
Guitart, C.
Auteure/Auteur
Redshaw, C.
Auteure/Auteur
van der Meer, J.R.
Auteure/Auteur
Readman, J.W.
Auteure/Auteur
Galloway, T.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0013-936X[print], 0013-936X[linking]
Statut éditorial
Publié
Date de publication
2009
Volume
43
Numéro
2
Première page
423
Dernière page/numéro d’article
428
Peer-reviewed
Oui
Langue
anglais
Résumé
Bacterial bioreporters have substantial potential for contaminant assessment but their real world application is currently impaired by a lack of sensitivity. Here, we exploit the bioconcentration of chemicals in the urine of animals to facilitate pollutant detection. The shore crab Carcinus maenas was exposed to the organic contaminant 2-hydroxybiphenyl, and urine was screened using an Escherichia coli-based luciferase gene (luxAB) reporter assay specific to this compound. Bioassay measurements differentiated between the original contaminant and its metabolites, quantifying bioconcentration factors of up to one hundred-fold in crab urine. Our results reveal the substantial potential of using bacterial bioreporter assays in real-time monitoring of biological matricesto determine exposure histories, with wide ranging potential for the in situ measurement of xenobiotics in risk assessments and epidemiology.
PID Serval
serval:BIB_F6C5CA5221DE
PMID
Date de création
2010-01-13T12:23:52.822Z
Date de création dans IRIS
2025-05-21T05:39:05Z