Titre
Novel Approach Combining Transcriptional and Evolutionary Signatures to Identify New Multiciliation Genes.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Defosset, A.
Auteure/Auteur
Merlat, D.
Auteure/Auteur
Poidevin, L.
Auteure/Auteur
Nevers, Y.
Auteure/Auteur
Kress, A.
Auteure/Auteur
Poch, O.
Auteure/Auteur
Lecompte, O.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
2073-4425
Statut éditorial
Publié
Date de publication
2021-09-21
Volume
12
Numéro
9
Première page
1452
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Publication Status: epublish
Résumé
Multiciliogenesis is a complex process that allows the generation of hundreds of motile cilia on the surface of specialized cells, to create fluid flow across epithelial surfaces. Dysfunction of human multiciliated cells is associated with diseases of the brain, airway and reproductive tracts. Despite recent efforts to characterize the transcriptional events responsible for the differentiation of multiciliated cells, a lot of actors remain to be identified. In this work, we capitalize on the ever-growing quantity of high-throughput data to search for new candidate genes involved in multiciliation. After performing a large-scale screening using 10 transcriptomics datasets dedicated to multiciliation, we established a specific evolutionary signature involving Otomorpha fish to use as a criterion to select the most likely targets. Combining both approaches highlighted a list of 114 potential multiciliated candidates. We characterized these genes first by generating protein interaction networks, which showed various clusters of ciliated and multiciliated genes, and then by computing phylogenetic profiles. In the end, we selected 11 poorly characterized genes that seem like particularly promising multiciliated candidates. By combining functional and comparative genomics methods, we developed a novel type of approach to study biological processes and identify new promising candidates linked to that process.
PID Serval
serval:BIB_F7D3952B2536
PMID
Open Access
Oui
Date de création
2021-10-04T09:09:32.053Z
Date de création dans IRIS
2025-05-21T05:40:25Z
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Nom
34573434_BIB_F7D3952B2536.pdf
Version du manuscrit
preprint
Licence
https://creativecommons.org/licenses/by/4.0
Taille
6.31 MB
Format
Adobe PDF
PID Serval
serval:BIB_F7D3952B2536.P001
Somme de contrôle
(MD5):cbc7d51933ba8a6c1f3a715170f713e0