Titre
Use of transgenic mice for the characterization of human alpha 1-acid glycoprotein (orosomucoid) variants.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Tomei, L.
Auteure/Auteur
Eap, C.B.
Auteure/Auteur
Baumann, P.
Auteure/Auteur
Dente, L.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0340-6717
Statut éditorial
Publié
Date de publication
1989
Volume
84
Numéro
1
Première page
89
Dernière page/numéro d’article
91
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
Sera from transgenic mice (TM) carrying human genes of alpha 1-acid glycoprotein (orosomucoid or ORM) have been analyzed by isoelectrofocusing and subsequent immunoblotting with antihuman ORM antibodies. With this technique it is possible to reveal selectively the human protein secreted in the TM sera. Orosomucoid bands present in TM sera have been compared with those of the most common human ORM phenotypes to correlate the products of specific genes to previously identified genetic variants. In this paper, we report the identification of the genes encoding for variants ORM1 F1 and ORM2 A, which are genes AGP-A and AGP-B/B' respectively. The nucleotide sequences of these genes are known; therefore a direct correlation between variants and specific amino acid sequences can be established.
PID Serval
serval:BIB_A2B706911F07
PMID
Date de création
2013-03-01T09:04:44.808Z
Date de création dans IRIS
2025-05-20T21:09:50Z