Titre
The Opto-inflammasome in zebrafish as a tool to study cell and tissue responses to speck formation and cell death.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Hasel de Carvalho, E.
Auteure/Auteur
Dharmadhikari, S.S.
Auteure/Auteur
Shkarina, K.
Auteure/Auteur
Xiong, J.R.
Auteure/Auteur
Reversade, B.
Auteure/Auteur
Broz, P.
Auteure/Auteur
Leptin, M.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
2050-084X
Statut éditorial
Publié
Date de publication
2023-07-07
Volume
12
Première page
e86373
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Publication Status: epublish
Résumé
The inflammasome is a conserved structure for the intracellular detection of danger or pathogen signals. As a large intracellular multiprotein signaling platform, it activates downstream effectors that initiate a rapid necrotic programmed cell death (PCD) termed pyroptosis and activation and secretion of pro-inflammatory cytokines to warn and activate surrounding cells. However, inflammasome activation is difficult to control experimentally on a single-cell level using canonical triggers. We constructed Opto-ASC, a light-responsive form of the inflammasome adaptor protein ASC (Apoptosis-Associated Speck-Like Protein Containing a CARD) which allows tight control of inflammasome formation in vivo. We introduced a cassette of this construct under the control of a heat shock element into zebrafish in which we can now induce ASC inflammasome (speck) formation in individual cells of the skin. We find that cell death resulting from ASC speck formation is morphologically distinct from apoptosis in periderm cells but not in basal cells. ASC-induced PCD can lead to apical or basal extrusion from the periderm. The apical extrusion in periderm cells depends on Caspb and triggers a strong Ca <sup>2+</sup> signaling response in nearby cells.
PID Serval
serval:BIB_D9A90FC8CC5C
PMID
Open Access
Oui
Date de création
2023-07-10T12:52:08.387Z
Date de création dans IRIS
2025-05-21T02:02:35Z
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Nom
37417734_BIB_D9A90FC8CC5C.pdf
Version du manuscrit
preprint
Licence
https://creativecommons.org/licenses/by/4.0
Taille
19.63 MB
Format
Adobe PDF
PID Serval
serval:BIB_D9A90FC8CC5C.P001
URN
urn:nbn:ch:serval-BIB_D9A90FC8CC5C1
Somme de contrôle
(MD5):0d5a5c46bffd85bc9e51067392ad350d