Titre
Altered hepatic transport of immunoglobulin A in mice lacking the J chain.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Hendrickson, B.A.
Auteure/Auteur
Conner, D.A.
Auteure/Auteur
Ladd, D.J.
Auteure/Auteur
Kendall, D.
Auteure/Auteur
Casanova, J.E.
Auteure/Auteur
Corthesy, B.
Auteure/Auteur
Max, E.E.
Auteure/Auteur
Neutra, M.R.
Auteure/Auteur
Seidman, C.E.
Auteure/Auteur
Seidman, J.G.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0022-1007
Statut éditorial
Publié
Date de publication
1995-12-01
Volume
182
Numéro
6
Première page
1905
Dernière page/numéro d’article
1911
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Publication Status: ppublish
Résumé
We have created J chain knockout mice to define the physiologic role of the J chain in immunoglobulin synthesis and transport. The J chain is covalently associated with pentameric immunoglobulin (Ig) M and dimeric IgA and is also expressed in most IgG-secreting cells. J chain-deficient mice have normal serum IgM and IgG levels but markedly elevated serum IgA. Although polymeric IgA was present in the mutant mice, a larger proportion of their serum IgA was monomeric than was found in wild-type mouse serum. Bile and fecal IgA levels were decreased in J chain-deficient mice compared with wild-type mice, suggesting inefficient transport of J chain-deficient IgA by hepatic polymeric immunoglobulin receptors (pIgR). The pIgR-mediated transport of serum-derived IgA from wild-type and mutant mice was assessed in Madin-Darby canine kidney (MDCK) cells transfected with the pIgR. These studies revealed selective transport by pIgR-expressing MDCK cells of wild-type IgA but not J chain-deficient IgA. We conclude that although the J chain is not required for IgA dimerization, it does affect the efficiency of polymerization or have a role in maintaining IgA dimer stability. Furthermore, the J chain is essential for efficient hepatic pIgR transport of IgA.
Sujets
PID Serval
serval:BIB_A97DE46A8D0C
PMID
Open Access
Oui
Date de création
2008-01-25T13:53:10.904Z
Date de création dans IRIS
2025-05-21T04:55:46Z
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7500036_BIB_A97DE46A8D0C.pdf
Version du manuscrit
published
Taille
934.13 KB
Format
Adobe PDF
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