Titre
Phosphate deficiency alters transcript isoforms via alternative transcription start sites.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Reis, R.S.
Auteure/Auteur
Clúa, J.
Auteure/Auteur
Jaskolowski, A.
Auteure/Auteur
Deforges, J.
Auteure/Auteur
Jacques-Vuarambon, D.
Auteure/Auteur
Guex, N.
Auteure/Auteur
Poirier, Y.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1365-313X
Statut éditorial
Publié
Date de publication
2024-10
Volume
120
Numéro
1
Première page
218
Dernière page/numéro d’article
233
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Résumé
Alternative transcription start sites (TSS) are widespread in eukaryotes and can alter the 5' UTR length and coding potential of transcripts. Here we show that inorganic phosphate (Pi) availability regulates the usage of several alternative TSS in Arabidopsis (Arabidopsis thaliana). In comparison to phytohormone treatment, Pi had a pronounced and specific effect on the usage of many alternative TSS. By combining short-read RNA sequencing with long-read sequencing of full-length mRNAs, we identified a set of 45 genes showing alternative TSS under Pi deficiency. Alternative TSS affected several processes, such as translation via the exclusion of upstream open reading frames present in the 5' UTR of RETICULAN LIKE PROTEIN B1 mRNA, and subcellular localization via removal of the plastid transit peptide coding region from the mRNAs of HEME OXYGENASE 1 and SULFOQUINOVOSYLDIACYLGLYCEROL 2. Several alternative TSS also generated shorter transcripts lacking the coding potential for important domains. For example, the EVOLUTIONARILY CONSERVED C-TERMINAL REGION 4 (ECT4) locus, which encodes an N <sup>6</sup> -methyladenosine (m <sup>6</sup> A) reader, strongly expressed under Pi deficiency a short noncoding transcript (named ALT <sub>ECT4</sub> ) ~550 nt long with a TSS in the penultimate intron. The specific and robust induction of ALT <sub>ECT4</sub> production by Pi deficiency led to the identification of a role for m <sup>6</sup> A readers in primary root growth in response to low phosphate that is dependent on iron and is involved in modulating cell division in the root meristem. Our results identify alternative TSS usage as an important process in the plant response to Pi deficiency.
Sujets
PID Serval
serval:BIB_DFA16258AAE3
PMID
Open Access
Oui
Date de création
2024-08-26T09:48:45.160Z
Date de création dans IRIS
2025-05-21T07:07:36Z
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Nom
39164918 .pdf
Version du manuscrit
published
Licence
https://creativecommons.org/licenses/by-nc-nd/4.0
Taille
2.11 MB
Format
Adobe PDF
PID Serval
serval:BIB_DFA16258AAE3.P001
URN
urn:nbn:ch:serval-BIB_DFA16258AAE33
Somme de contrôle
(MD5):a4281c04b7e710a119b90704033bc3c5