Titre
Mechanical characterization of collagen fibers and scaffolds for tissue engineering.
Type
article
Institution
Externe
Périodique
Auteur(s)
Gentleman, E.
Auteure/Auteur
Lay, A.N.
Auteure/Auteur
Dickerson, D.A.
Auteure/Auteur
Nauman, E.A.
Auteure/Auteur
Livesay, G.A.
Auteure/Auteur
Dee, K.C.
Auteure/Auteur
Liens vers les personnes
ISSN
0142-9612
Statut éditorial
Publié
Date de publication
2003-09
Volume
24
Numéro
21
Première page
3805
Dernière page/numéro d’article
3813
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
Publication Status: ppublish
Publication Status: ppublish
Résumé
Engineered tissues must utilize scaffolding biomaterials that support desired cellular functions and possess or can develop appropriate mechanical characteristics. This study assessed properties of collagen as a scaffolding biomaterial for ligament replacements. Mechanical properties of extruded bovine achilles tendon collagen fibers were significantly affected by fiber diameter, with smaller fibers displaying higher tangent moduli and peak stresses. Mechanical properties of 125 micrometer-diameter extruded fibers (tangent modulus of 359.6+/-28.4MPa; peak stress of 36.0+/-5.4MPa) were similar to properties reported for human ligaments. Scaffolds of extruded fibers did not exhibit viscoelastic creep properties similar to natural ligaments. Collagen fibers from rat tail tendon (a well-studied comparison material) displayed characteristic strain-softening behavior, and scaffolds of rat tail fibers demonstrated a non-intuitive relationship between tangent modulus and specimen length. Composite scaffolds (extruded collagen fibers cast within a gel of Type I rat tail tendon collagen) were maintained with and without fibroblasts under standard culture conditions for 25 days; cell-incorporated scaffolds displayed significantly higher tangent moduli and peak stresses than those without cells. Because tissue-engineered products must possess appropriate mechanical as well as biological/chemical properties, data from this study should help enable the development of improved tissue analogues.
PID Serval
serval:BIB_6FFEDAB15980
PMID
Date de création
2024-01-12T09:14:26.087Z
Date de création dans IRIS
2025-05-21T00:58:36Z