Titre
Biochemical differentiation of mechanically dissociated mammalian brain in aggregating cell culture.
Type
article
Institution
Externe
Périodique
Auteur(s)
Honegger, P.
Auteure/Auteur
Richelson, E.
Auteure/Auteur
Liens vers les personnes
ISSN
0006-8993
Statut éditorial
Publié
Date de publication
1976
Volume
109
Numéro
2
Première page
335
Dernière page/numéro d’article
354
Langue
anglais
Résumé
Mouse and rat brain cells were dissociated by a simple mechanical sieving technique and studied in culture for the formation of aggregates and the activities of choline acetyltransferase, acetylcholinesterase, glutamic acid decarboxylase, tyrosine 3-monooxygenase, aromatic L-amino acid decarboxylase, catechol methyltransferase, and monoamine oxidase. Cells from fetal and neonatal tissue formed aggregates but not cells from tissue older than two days after birth. The pattern of development of enzyme activities in these aggregates varied with the age of starting tissue. The highest levels of specific activity for the neuron-specific enzymes were found after 3-4 weeks in culture for aggregates of cells derived from relatively undeveloped brains.
Sujets
PID Serval
serval:BIB_B67590F8BDC0
PMID
Date de création
2008-01-24T12:12:07.681Z
Date de création dans IRIS
2025-05-20T23:17:28Z