Titre
Distinct expression levels and patterns of stem cell marker, aldehyde dehydrogenase isoform 1 (ALDH1), in human epithelial cancers.
Type
article
Institution
Externe
Périodique
Auteur(s)
Deng, S.
Auteure/Auteur
Yang, X.
Auteure/Auteur
Lassus, H.
Auteure/Auteur
Liang, S.
Auteure/Auteur
Kaur, S.
Auteure/Auteur
Ye, Q.
Auteure/Auteur
Li, C.
Auteure/Auteur
Wang, L.P.
Auteure/Auteur
Roby, K.F.
Auteure/Auteur
Orsulic, S.
Auteure/Auteur
Connolly, D.C.
Auteure/Auteur
Zhang, Y.
Auteure/Auteur
Montone, K.
Auteure/Auteur
Bützow, R.
Auteure/Auteur
Coukos, G.
Auteure/Auteur
Zhang, L.
Auteure/Auteur
Liens vers les personnes
ISSN
1932-6203
Statut éditorial
Publié
Date de publication
2010
Volume
5
Numéro
4
Première page
e10277
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.Publication Status: epublish
Résumé
Aldehyde dehydrogenase isoform 1 (ALDH1) has been proved useful for the identification of cancer stem cells. However, our knowledge of the expression and activity of ALDH1 in common epithelial cancers and their corresponding normal tissues is still largely absent. Therefore, we characterized ALDH1 expression in 24 types of normal tissues and a large collection of epithelial tumor specimens (six cancer types, n = 792) by immunohistochemical staining. Using the ALDEFUOR assay, ALDH1 activity was also examined in 16 primary tumor specimens and 43 established epithelial cancer cell lines. In addition, an ovarian cancer transgenic mouse model and 7 murine ovarian cancer cell lines were analyzed. We found that the expression levels and patterns of ALDH1 in epithelial cancers are remarkably distinct, and they correlate with their corresponding normal tissues. ALDH1 protein expression levels are positively correlated with ALDH1 enzymatic activity measured by ALDEFLUOR assay. Long-term in vitro culture doesn't significantly affect ALDH1 activity in epithelial tumor cells. Consistent with research on other cancers, we found that high ALDH1 expression is significantly associated with poor clinical outcomes in serous ovarian cancer patients (n = 439, p = 0.0036). Finally, ALDH(br) tumor cells exhibit cancer stem cell properties and are resistant to chemotherapy. As a novel cancer stem cell marker, ALDH1 can be used for tumors whose corresponding normal tissues express ALDH1 in relatively restricted or limited levels such as breast, lung, ovarian or colon cancer.
PID Serval
serval:BIB_A2EEE76F823E
PMID
Open Access
Oui
Date de création
2014-10-14T10:42:47.187Z
Date de création dans IRIS
2025-05-21T01:51:58Z