Titre
Unraveling cartilage degeneration through synergistic effects of hydrostatic pressure and biomimetic temperature increase.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Guo, Y.
Auteure/Auteur
Stampoultzis, T.
Auteure/Auteur
Nasrollahzadeh, N.
Auteure/Auteur
Karami, P.
Auteure/Auteur
Rana, V.K.
Auteure/Auteur
Applegate, L.
Auteure/Auteur
Pioletti, D.P.
Auteure/Auteur
Liens vers les unités
ISSN
2589-0042
Statut éditorial
Publié
Date de publication
2023-12-15
Volume
26
Numéro
12
Première page
108519
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Publication Status: epublish
Résumé
Cartilage degeneration, typically viewed as an irreversible, vicious cycle, sees a significant reduction in two essential biophysical cues: the well-established hydrostatic pressure (HP) and the recently discovered transient temperature increase. Our study aimed to evaluate the combined influence of these cues on maintaining cartilage homeostasis. To achieve this, we developed a customized bioreactor, designed to mimic the specific hydrostatic pressure and transient thermal increase experienced during human knee physiological activities. This system enabled us to investigate the response of human 3D-cultured chondrocytes and human cartilage explants to either isolated or combined hydrostatic pressure and thermal stimuli. Our study found that chondroinduction (SOX9, aggrecan, and sulfated glycosaminoglycan) and chondroprotection (HSP70) reached maximum expression levels when hydrostatic pressure and transient thermal increase acted in tandem, underscoring the critical role of these combined cues in preserving cartilage homeostasis. These findings led us to propose a refined model of the vicious cycle of cartilage degeneration.
PID Serval
serval:BIB_3D5ADEF0B7F4
PMID
Open Access
Oui
Date de création
2024-01-10T10:19:34.598Z
Date de création dans IRIS
2025-05-20T13:27:33Z
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Nom
38125014_BIB_3D5ADEF0B7F4.pdf
Version du manuscrit
published
Licence
https://creativecommons.org/licenses/by-nc-nd/4.0
Taille
4.59 MB
Format
Adobe PDF
PID Serval
serval:BIB_3D5ADEF0B7F4.P001
URN
urn:nbn:ch:serval-BIB_3D5ADEF0B7F45
Somme de contrôle
(MD5):be755f59da0d145db5577c434de7e606