Titre

Evaluation of uptake and transport of cationic and anionic ultrasmall iron oxide nanoparticles by human colon cells.

Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Kenzaoui, B.H.
Auteure/Auteur
Vilà, M.R.
Auteure/Auteur
Miquel, J.M.
Auteure/Auteur
Cengelli, F.
Auteure/Auteur
Juillerat-Jeanneret, L.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1178-2013
Statut éditorial
Publié
Date de publication
2012
Volume
7
Première page
1275
Dernière page/numéro d’article
1286
Langue
anglais
Notes
Publication types: Journal Article
Résumé
Nanoparticles (NPs) are in clinical use or under development for therapeutic imaging and drug delivery. However, relatively little information exists concerning the uptake and transport of NPs across human colon cell layers, or their potential to invade three-dimensional models of human colon cells that better mimic the tissue structures of normal and tumoral colon. In order to gain such information, the interactions of biocompatible ultrasmall superparamagnetic iron oxide nanoparticles (USPIO NPs) (iron oxide core 9-10 nm) coated with either cationic polyvinylamine (aminoPVA) or anionic oleic acid with human HT-29 and Caco-2 colon cells was determined. The uptake of the cationic USPIO NPs was much higher than the uptake of the anionic USPIO NPs. The intracellular localization of aminoPVA USPIO NPs was confirmed in HT-29 cells by transmission electron microscopy that detected the iron oxide core. AminoPVA USPIO NPs invaded three-dimensional spheroids of both HT-29 and Caco-2 cells, whereas oleic acid-coated USPIO NPs could only invade Caco-2 spheroids. Neither cationic aminoPVA USPIO NPs nor anionic oleic acid-coated USPIO NPs were transported at detectable levels across the tight CacoReady? intestinal barrier model or the more permeable mucus-secreting CacoGoblet? model.
PID Serval
serval:BIB_9F63AD8A9ED0
DOI
PMID
WOS
Permalien
Open Access
Oui
Date de création
2012-05-05T14:59:56.199Z
Date de création dans IRIS
2025-05-21T03:17:54Z
Fichier(s)
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Vignette d'image
Nom

BIB_9F63AD8A9ED0.P001.pdf

Version du manuscrit

preprint

Taille

5.2 MB

Format

Adobe PDF

PID Serval

serval:BIB_9F63AD8A9ED0.P001

URN

urn:nbn:ch:serval-BIB_9F63AD8A9ED02

Somme de contrôle

(MD5):b8c10dfd23759227a1ba1669fe0e7e09