Titre
Systemic antibody responses to gut commensal bacteria during chronic HIV-1 infection.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Haas, A.
Auteure/Auteur
Zimmermann, K.
Auteure/Auteur
Graw, F.
Auteure/Auteur
Slack, E.
Auteure/Auteur
Rusert, P.
Auteure/Auteur
Ledergerber, B.
Auteure/Auteur
Bossart, W.
Auteure/Auteur
Weber, R.
Auteure/Auteur
Thurnheer, M.C.
Auteure/Auteur
Battegay, M.
Auteure/Auteur
Hirschel, B.
Auteure/Auteur
Vernazza, P.
Auteure/Auteur
Patuto, N.
Auteure/Auteur
Macpherson, A.J.
Auteure/Auteur
Günthard, H.F.
Auteure/Auteur
Oxenius, A.
Auteure/Auteur
Contributrices/contributeurs
Barth, J.
Battegay, M.
Bernasconi, E.
Boni, J.
Brazzola, P.
Bucher, HC.
Burgisser, P.
Burton-Jeangros, C.
Calmy, A.
Cavassini, M.
Cheseaux, JJ.
Drack, G.
Dubs, R.
Duppenthaler, A.
Egger, M.
Elzi, L.
Fehr, J.
Fischer, M.
Flepp, M.
Francioli, P.
Furrer, H.
Fux, CA.
Gorgievski, M.
Grawe, C.
Gunthard, H.
Gyr, T.
Hasse, B.
Kahlert, C.
Hirsch, HH.
Hirschel, B.
Hosli, I.
Kahlert, C.
Kaiser, L.
Keiser, O.
Kind, C.
Klimkait, T.
Kovari, H.
Ledergerber, B.
Martinetti, G.
Martinez de Tejada, B.
Muller, N.
Nadal, D.
Pantaleo, G.
Posfay-Barbe, K.
Raio, L.
Rauch, A.
Regenass, S.
Rickenbach, M.
Rudin, C.
Schmid, P.
Schultze, D.
Schoni-Affolter, F.
Schupbach, J.
Speck, R.
Taffe, P.
Telenti, A.
Trkola, A.
Vernazza, P.
von Wyl, V.
Weber, R.
Wyler, CA.
Yerly, S.
Groupes de travail
Swiss HIV Cohort Study
Liens vers les personnes
Liens vers les unités
ISSN
1468-3288
Statut éditorial
Publié
Date de publication
2011
Volume
60
Numéro
11
Première page
1506
Dernière page/numéro d’article
1519
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Résumé
BACKGROUND: Human systemic antibody responses to commensal microbiota are not well characterised during health and disease. Of particular interest is the analysis of their potential modulation caused by chronic HIV-1 infection which is associated with sustained enteropathy and systemic B cell disturbances reflected by impaired B cell responses and chronic B cell hyperactivity. The mechanisms underlying B cell hyperactivation and the specificities of the resulting hypergammaglobulinaemia are only poorly understood.
METHODS: By a technique referred to as live bacterial FACS (fluorescence-activated cell sorting), the present study investigated systemic antibody responses to several gut and skin commensal bacteria as well as Candida albicans in longitudinal plasma and serum samples from healthy donors, chronic HIV-1-infected individuals with or without diarrhoea and patients with inflammatory bowel disease (IBD).
RESULTS: The data show that systemic antibody responses to the commensal microbiota were abundantly present in humans and remained remarkably stable over years. Overall systemic antibody responses to gut commensal bacteria were not affected during chronic HIV-1 infection, with titres decreasing when normalised to elevated plasma immunoglobulin G (IgG) levels found in patients with HIV. In contrast, increases in the titres of high affinity antimicrobiota antibodies were detected in patients with IBD, demonstrating that conditions with known increased intestinal permeability and aberrant mutualism can induce changes in antibody titres observed in these assays.
CONCLUSION: Neither HIV-associated enteropathy nor B cell dysfunction impact on the high-affinity systemic antibody responses to gut commensal bacteria. HIV-associated hypergammaglobulinaemia is therefore unlikely to be driven by induction of antimicrobiota antibodies.
METHODS: By a technique referred to as live bacterial FACS (fluorescence-activated cell sorting), the present study investigated systemic antibody responses to several gut and skin commensal bacteria as well as Candida albicans in longitudinal plasma and serum samples from healthy donors, chronic HIV-1-infected individuals with or without diarrhoea and patients with inflammatory bowel disease (IBD).
RESULTS: The data show that systemic antibody responses to the commensal microbiota were abundantly present in humans and remained remarkably stable over years. Overall systemic antibody responses to gut commensal bacteria were not affected during chronic HIV-1 infection, with titres decreasing when normalised to elevated plasma immunoglobulin G (IgG) levels found in patients with HIV. In contrast, increases in the titres of high affinity antimicrobiota antibodies were detected in patients with IBD, demonstrating that conditions with known increased intestinal permeability and aberrant mutualism can induce changes in antibody titres observed in these assays.
CONCLUSION: Neither HIV-associated enteropathy nor B cell dysfunction impact on the high-affinity systemic antibody responses to gut commensal bacteria. HIV-associated hypergammaglobulinaemia is therefore unlikely to be driven by induction of antimicrobiota antibodies.
Sujets
PID Serval
serval:BIB_2A28B30FEEFB
PMID
Date de création
2011-10-21T09:07:35.786Z
Date de création dans IRIS
2025-05-20T15:21:19Z