Titre
The heat shock response in moss plants is regulated by specific calcium-permeable channels in the plasma membrane.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Saidi, Y.
Auteure/Auteur
Finka, A.
Auteure/Auteur
Muriset, M.
Auteure/Auteur
Bromberg, Z.
Auteure/Auteur
Weiss, Y.G.
Auteure/Auteur
Maathuis, F.J.
Auteure/Auteur
Goloubinoff, P.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1532-298X[electronic], 1040-4651[linking]
Statut éditorial
Publié
Date de publication
2009
Volume
21
Numéro
9
Première page
2829
Dernière page/numéro d’article
2843
Peer-reviewed
Oui
Langue
anglais
Résumé
Land plants are prone to strong thermal variations and must therefore sense early moderate temperature increments to induce appropriate cellular defenses, such as molecular chaperones, in anticipation of upcoming noxious temperatures. To investigate how plants perceive mild changes in ambient temperature, we monitored in recombinant lines of the moss Physcomitrella patens the activation of a heat-inducible promoter, the integrity of a thermolabile enzyme, and the fluctuations of cytoplasmic calcium. Mild temperature increments, or isothermal treatments with membrane fluidizers or Hsp90 inhibitors, induced a heat shock response (HSR) that critically depended on a preceding Ca(2+) transient through the plasma membrane. Electrophysiological experiments revealed the presence of a Ca(2+)-permeable channel in the plasma membrane that is transiently activated by mild temperature increments or chemical perturbations of membrane fluidity. The amplitude of the Ca(2+) influx during the first minutes of a temperature stress modulated the intensity of the HSR, and Ca(2+) channel blockers prevented HSR and the onset of thermotolerance. Our data suggest that early sensing of mild temperature increments occurs at the plasma membrane of plant cells independently from cytosolic protein unfolding. The heat signal is translated into an effective HSR by way of a specific membrane-regulated Ca(2+) influx, leading to thermotolerance.
Sujets
PID Serval
serval:BIB_7741FF7DABD6
PMID
Open Access
Oui
Date de création
2009-11-23T12:20:23.058Z
Date de création dans IRIS
2025-05-21T01:33:25Z