Titre
Highly diverse TCRα chain repertoire of pre-immune CD8(+) T cells reveals new insights in gene recombination.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Genolet, R.
Auteure/Auteur
Stevenson, B.J.
Auteure/Auteur
Farinelli, L.
Auteure/Auteur
Osterås, M.
Auteure/Auteur
Luescher, I.F.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1460-2075
Statut éditorial
Publié
Date de publication
2012
Volume
31
Numéro
7
Première page
1666
Dernière page/numéro d’article
1678
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Résumé
Although the T-cell receptor αδ (TCRαδ) locus harbours large libraries of variable (TRAV) and junctional (TRAJ) gene segments, according to previous studies the TCRα chain repertoire is of limited diversity due to restrictions imposed by sequential coordinate TRAV-TRAJ recombinations. By sequencing tens of millions of TCRα chain transcripts from naive mouse CD8(+) T cells, we observed a hugely diverse repertoire, comprising nearly all possible TRAV-TRAJ combinations. Our findings are not compatible with sequential coordinate gene recombination, but rather with a model in which contraction and DNA looping in the TCRαδ locus provide equal access to TRAV and TRAJ gene segments, similarly to that demonstrated for IgH gene recombination. Generation of the observed highly diverse TCRα chain repertoire necessitates deletion of failed attempts by thymic-positive selection and is essential for the formation of highly diverse TCRαβ repertoires, capable of providing good protective immunity.
PID Serval
serval:BIB_2B947DF61C00
PMID
Open Access
Oui
Date de création
2012-05-05T14:33:32.588Z
Date de création dans IRIS
2025-05-20T19:15:39Z