Titre
Evaluation of the impact of iPSC differentiation protocols on transcriptomic signatures.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Chandrasekaran, V.
Auteure/Auteur
Wellens, S.
Auteure/Auteur
Bourguignon, A.
Auteure/Auteur
Djidrovski, I.
Auteure/Auteur
Fransen, L.
Auteure/Auteur
Ghosh, S.
Auteure/Auteur
Mazidi, Z.
Auteure/Auteur
Murphy, C.
Auteure/Auteur
Nunes, C.
Auteure/Auteur
Singh, P.
Auteure/Auteur
Zana, M.
Auteure/Auteur
Armstrong, L.
Auteure/Auteur
Dinnyés, A.
Auteure/Auteur
Grillari, J.
Auteure/Auteur
Grillari-Voglauer, R.
Auteure/Auteur
Leonard, M.O.
Auteure/Auteur
Verfaillie, C.
Auteure/Auteur
Wilmes, A.
Auteure/Auteur
Zurich, M.G.
Auteure/Auteur
Exner, T.
Auteure/Auteur
Jennings, P.
Auteure/Auteur
Culot, M.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1879-3177
Statut éditorial
Publié
Date de publication
2024-06
Volume
98
Première page
105826
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Résumé
Human induced pluripotent stem cells (iPSC) have the potential to produce desired target cell types in vitro and allow for the high-throughput screening of drugs/chemicals at population level thereby minimising the cost of drug discovery and drug withdrawals after clinical trials. There is a substantial need for the characterisation of the iPSC derived models to better understand and utilise them for toxicological relevant applications. In our study, iPSC (SBAD2 or SBAD3 lines obtained from StemBANCC project) were differentiated towards toxicologically relevant cell types: alveolar macrophages, brain capillary endothelial cells, brain cells, endothelial cells, hepatocytes, lung airway epithelium, monocytes, podocytes and renal proximal tubular cells. A targeted transcriptomic approach was employed to understand the effects of differentiation protocols on these cell types. Pearson correlation and principal component analysis (PCA) separated most of the intended target cell types and undifferentiated iPSC models as distinct groups with a high correlation among replicates from the same model. Based on PCA, the intended target cell types could also be separated into the three germ layer groups (ectoderm, endoderm and mesoderm). Differential expression analysis (DESeq2) presented the upregulated genes in each intended target cell types that allowed the evaluation of the differentiation to certain degree and the selection of key differentiation markers. In conclusion, these data confirm the versatile use of iPSC differentiated cell types as standardizable and relevant model systems for in vitro toxicology.
PID Serval
serval:BIB_17CE74341EA3
PMID
Open Access
Oui
Date de création
2024-04-19T07:04:16.636Z
Date de création dans IRIS
2025-05-20T18:13:16Z
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Nom
38615723.pdf
Version du manuscrit
published
Licence
https://creativecommons.org/licenses/by/4.0
Taille
6.23 MB
Format
Adobe PDF
PID Serval
serval:BIB_17CE74341EA3.P001
URN
urn:nbn:ch:serval-BIB_17CE74341EA35
Somme de contrôle
(MD5):4931bc239a443c9fec9fd67148436723