Titre
Transport of tetraethylammonium by a kidney epithelial cell line (LLC-PK1)
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
American journal of physiology. Renal, fluid and electrolyte physiology
Auteur(s)
Fauth, C.
Rossier, B.
Roch-Ramel, F.
Liens vers les personnes
Liens vers les unités
ISSN
0363-6127
Statut éditorial
Publié
Date de publication
1988-03
Volume
254
Numéro
3 Pt 2
Première page
F351
Dernière page/numéro d’article
7
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar
Research Support, Non-U.S. Gov't --- Old month value: Mar
Résumé
We investigated whether the LLC-PK1 epithelial cell line (which shows many characteristics of proximal tubular cells) also is capable of transporting an organic ion. Suspended LLC-PK1 cells accumulated tetraethylammonium (TEA). The uptake showed characteristics of a facilitated mechanism; TEA uptake was saturable and temperature-dependent and was inhibited by other organic cations. Quinine and mepiperphenidol were the most potent inhibitors, whereas N1-methylnicotinamide and morphine inhibited the transport system only slightly at doses of 10(-3) M. Basolateral-to-apical TEA flux through LLC-PK1 monolayers was five to six times larger than that of mannitol, a nontransported compound, whereas apical-to-basolateral TEA and mannitol fluxes were equal. Only the basolateral-to-apical TEA flux was inhibited by quinine. Under similar experimental conditions, no transport of p-aminohippuric acid was observed. It is concluded that LLC-PK1 cells are able to transport TEA, as do cells of the proximal tubule.
Sujets
PID Serval
serval:BIB_65767F8D7940
PMID
Date de création
2008-01-24T12:00:22.368Z
Date de création dans IRIS
2025-05-20T17:04:10Z