Titre
A TLR7/8 Agonist-Including DOEPC-Based Cationic Liposome Formulation Mediates Its Adjuvanticity Through the Sustained Recruitment of Highly Activated Monocytes in a Type I IFN-Independent but NF-κB-Dependent Manner.
Type
article
Institution
Externe
Périodique
Auteur(s)
Auderset, F.
Auteure/Auteur
Belnoue, E.
Auteure/Auteur
Mastelic-Gavillet, B.
Auteure/Auteur
Lambert, P.H.
Auteure/Auteur
Siegrist, C.A.
Auteure/Auteur
Liens vers les personnes
ISSN
1664-3224
Statut éditorial
Publié
Date de publication
2020
Volume
11
Première page
580974
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Publication Status: epublish
Résumé
Novel adjuvants, such as Toll-like receptors (TLRs) agonists, are needed for the development of new formulations able to circumvent limitations of current vaccines. Among TLRs, TLR7/8 agonists represent promising candidates, as they are well described to enhance antigen-specific antibody responses and skew immunity toward T helper (T <sub>H</sub> ) 1 responses. We find here that the incorporation of the synthetic TLR7/8 ligand 3M-052 in a cationic DOEPC-based liposome formulation shifts immunity toward T <sub>H</sub> 1 responses and elicits strong and long-lasting germinal center and follicular T helper cell responses in adult mice. This reflects the prolonged recruitment of innate cells toward the site of immunization and homing of activated antigen-loaded monocytes and monocyte-derived dendritic cells toward draining lymph nodes. We further show that this adjuvanticity is independent of type I IFN but NF-κB-dependent. Overall, our data identify TLR7/8 agonists incorporated in liposomes as promising and effective adjuvants to enhance T <sub>H</sub> 1 and germinal center responses.
Sujets
PID Serval
serval:BIB_B3824234F961
PMID
Open Access
Oui
Date de création
2020-12-15T08:06:08.497Z
Date de création dans IRIS
2025-05-20T22:44:39Z