Titre
MOM22 is a receptor for mitochondrial targeting sequences and cooperates with MOM19.
Type
article
Institution
Externe
Périodique
Auteur(s)
Mayer, A.
Auteure/Auteur
Nargang, F.E.
Auteure/Auteur
Neupert, W.
Auteure/Auteur
Lill, R.
Auteure/Auteur
Liens vers les personnes
ISSN
0261-4189
Statut éditorial
Publié
Date de publication
1995
Volume
14
Numéro
17
Première page
4204
Dernière page/numéro d’article
4211
Peer-reviewed
Oui
Langue
anglais
Résumé
Recognition of targeting signals is a crucial step in protein sorting within the cell. So far, only a few components capable of deciphering targeting signals have been identified, and insights into the chemical nature of the interaction between the signals and their receptors are scarce. Using highly purified mitochondrial outer membrane vesicles, we demonstrate that MOM22 and MOM19, components of the protein import complex of the outer membrane, bind preproteins at the mitochondrial surface in a reversible fashion. Interaction specifically and directly occurs with the N-terminal presequence and is abolished after inactivation of either MOM22 or MOM19. Binding is salt sensitive, suggesting that recognition involves electrostatic forces between the positive charges of the presequence and the acidic cytosolic domain of MOM22. MOM19 and MOM22 can be cross-linked with high efficiency. We propose that the two proteins form a complex which functions as the presequence receptor at the mitochondrial surface and facilitates the movement of preproteins into the translocation pore.
Sujets
PID Serval
serval:BIB_A27F1B0DA2B9
PMID
Date de création
2008-01-24T14:06:13.824Z
Date de création dans IRIS
2025-05-20T21:42:52Z