Titre
The Waddlia genome: a window into chlamydial biology.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
PloS One
Auteur(s)
Bertelli, Claire
Auteure/Auteur
Collyn, Francois
Auteure/Auteur
Croxatto, Antony
Auteure/Auteur
Rueckert, Christian
Auteure/Auteur
Polkinghorne, Adam
Auteure/Auteur
Kebbi-Beghdadi, Carole
Auteure/Auteur
Goesmann, Alexander
Auteure/Auteur
Vaughan, Lloyd
Auteure/Auteur
Greub, Gilbert
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1932-6203[electronic], 1932-6203[linking]
Statut éditorial
Publié
Date de publication
2010
Volume
5
Numéro
5
Première page
10890
Langue
anglais
Résumé
Growing evidence suggests that a novel member of the Chlamydiales order, Waddlia chondrophila, is a potential agent of miscarriage in humans and abortion in ruminants. Due to the lack of genetic tools to manipulate chlamydia, genomic analysis is proving to be the most incisive tool in stimulating investigations into the biology of these obligate intracellular bacteria. 454/Roche and Solexa/Illumina technologies were thus used to sequence and assemble de novo the full genome of the first representative of the Waddliaceae family, W. chondrophila. The bacteria possesses a 2'116'312 bp chromosome and a 15'593 bp low-copy number plasmid that might integrate into the bacterial chromosome. The Waddlia genome displays numerous repeated sequences indicating different genome dynamics from classical chlamydia which almost completely lack repetitive elements. Moreover, W. chondrophila exhibits many virulence factors also present in classical chlamydia, including a functional type III secretion system, but also a large complement of specific factors for resistance to host or environmental stresses. Large families of outer membrane proteins were identified indicating that these highly immunogenic proteins are not Chlamydiaceae specific and might have been present in their last common ancestor. Enhanced metabolic capability for the synthesis of nucleotides, amino acids, lipids and other co-factors suggests that the common ancestor of the modern Chlamydiales may have been less dependent on their eukaryotic host. The fine-detailed analysis of biosynthetic pathways brings us closer to possibly developing a synthetic medium to grow W. chondrophila, a critical step in the development of genetic tools. As a whole, the availability of the W. chondrophila genome opens new possibilities in Chlamydiales research, providing new insights into the evolution of members of the order Chlamydiales and the biology of the Waddliaceae.
PID Serval
serval:BIB_D05727459AD1
PMID
Open Access
Oui
Date de création
2010-06-23T08:32:49.977Z
Date de création dans IRIS
2025-05-21T00:58:36Z
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Nom
BIB_D05727459AD1.P001.pdf
Version du manuscrit
preprint
Taille
2.57 MB
Format
Adobe PDF
PID Serval
serval:BIB_D05727459AD1.P001
URN
urn:nbn:ch:serval-BIB_D05727459AD10
Somme de contrôle
(MD5):37b8851ac08182242ef6a6a4a45e7264