Titre
Corticosterone induces 11 beta-HSD and mineralocorticoid specificity in an amphibian urinary bladder cell line
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Auteur(s)
Gaeggeler, H. P.
Auteure/Auteur
Duperrex, H.
Auteure/Auteur
Hautier, S.
Auteure/Auteur
Rossier, B. C.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0363-6143
Statut éditorial
Publié
Date de publication
1993-02
Volume
264
Numéro
2 Pt 1
Première page
C317
Dernière page/numéro d’article
22
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Feb
Research Support, Non-U.S. Gov't --- Old month value: Feb
Résumé
We have examined the mineralocorticoid specificity in a TBM 18-23 cell line derived from the toad bladder epithelium. In cells grown on porous substrate, corticosterone was more potent than aldosterone in stimulating a sodium transport response, measured by the short-circuit current method 6 h after hormone addition [mean affinity constant (K0.5) for corticosterone = 1 nM vs. K0.5 for aldosterone = 8 nM]. The time course of effects and saturation kinetics were identical for both agonists, suggesting interaction with identical receptors. Whereas the dose-response relationship for aldosterone did not change with time of incubation (6 vs. 24 h), the dose-response curve for corticosterone became biphasic at 24-h incubation (apparent K0.5 as high as 40 nM), demonstrating that corticosterone became apparently less potent with time. Pretreatment with carbenoxolone, a potent inhibitor of 11 beta-hydroxysteroid dehydrogenase (11 beta-HSD), restored full sensitivity at 24-h incubation to corticosterone. The 11 beta-HSD activity was low during the first 3 h of incubation in the presence of 3 nM corticosterone, and only a small fraction (approximately 7%) of corticosterone was metabolized. At 24-h incubation, 11 beta-HSD activity increased approximately 2.5-fold (P < 0.001, n = 8). We conclude that 11 beta-HSD activity is induced by its own substrate in TBM cells in parallel with the induction of the carbenoxolone-sensitive sodium transport response.
Sujets
PID Serval
serval:BIB_40266D21C0AC
PMID
Date de création
2008-01-24T12:00:20.134Z
Date de création dans IRIS
2025-05-20T18:56:54Z