Titre
Direct transfer of antigen-specific cytolytic activity to noncytolytic cells upon fusion with liposomes derived from cytolytic T cell clones.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Harris, D.T.
Auteure/Auteur
MacDonald, H.R.
Auteure/Auteur
Cerottini, J.C.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0022-1007
Statut éditorial
Publié
Date de publication
1984-01-01
Volume
159
Numéro
1
Première page
261
Dernière page/numéro d’article
275
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Résumé
Murine cytolytic T lymphocytes (CTL) clones were solubilized in Nonidet P-40 detergent, and the matrix and membrane proteins separated from the nuclear constituents. These proteins, in combination with exogenous lipids and Sendai virus envelope proteins, were used to construct liposomes that were then fused with noncytolytic cloned T cell recipients. The resultant fusion products were found to be highly cytolytic and appeared to express the same specificity as the original donor clone. Further analysis showed that the liposomal transfer process was extremely efficient. Moreover, in addition to noncytolytic T cell clones, three transformed T cell lines and one B cell line were found to express specific cytolytic activity after fusion with appropriate liposomes. Inhibition experiments using monoclonal antibodies against target cell antigens, as well as analysis of the lytic reactivity pattern of the fusion products, confirmed the high degree of specificity conferred to the recipient cells. This study thus indicates that the two characteristics typically associated with CTL, namely antigen-specific recognition and cytolytic activity, can be solubilized from CTL and transferred to recipient cells that do not express these characteristics.
Sujets
PID Serval
serval:BIB_2298B5B3A805
PMID
Open Access
Oui
Date de création
2008-01-28T10:14:09.911Z
Date de création dans IRIS
2025-05-20T15:52:21Z
Fichier(s)![Vignette d'image]()
En cours de chargement...
Nom
6198423_BIB_2298B5B3A805.pdf
Version du manuscrit
published
Taille
1.08 MB
Format
Adobe PDF
PID Serval
serval:BIB_2298B5B3A805.P001
Somme de contrôle
(MD5):a2c1766a574e478258f19f5876b25c60