Titre
The response of Mucor plumbeus to pentachlorophenol: a toxicoproteomics study.
Type
article
Institution
Externe
Périodique
Auteur(s)
Carvalho, M.B.
Auteure/Auteur
Martins, I.
Auteure/Auteur
Medeiros, J.
Auteure/Auteur
Tavares, S.
Auteure/Auteur
Planchon, S.
Auteure/Auteur
Renaut, J.
Auteure/Auteur
Núñez, O.
Auteure/Auteur
Gallart-Ayala, H.
Auteure/Auteur
Galceran, M.T.
Auteure/Auteur
Hursthouse, A.
Auteure/Auteur
Silva Pereira, C.
Auteure/Auteur
Liens vers les personnes
ISSN
1876-7737
Statut éditorial
Publié
Date de publication
2013-01-14
Volume
78
Première page
159
Dernière page/numéro d’article
171
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Résumé
Pentachlorophenol (PCP) represents a critical concern worldwide due to its toxicity and recalcitrance to degradation. The capacity of Mucor plumbeus to transform PCP into several detoxification metabolites, including tetrachlorohydroquinone and several phase II conjugates, was observed by LC-HRMS. The data obtained support the degradation pathway proposed previously. PCP effects in M. plumbeus, an unsequenced species, were investigated using a proteomics approach (bidimensional gel electrophoresis followed by MALDI TOF/TOF analyses). The mycelial proteins identified in the differentially accumulated spots allowed the identification of PCP responsive proteins. The presence of PCP increased the energy demand, altered the cell wall architecture and cytoskeleton and induced a significant stress response. The latter was emphasised by the up-accumulation of protein species associated with defence mechanisms (e.g. HSP70 and cytochrome c peroxidase). Overall the data produced corroborate the capability of PCP to uncouple oxidative-phosphorylation in mitochondria. Importantly, one of the identified mycelial protein species, a NAD- and Zn-dependent ADH, is likely to be involved in PCP degradation. Amongst the fungal secretome analysed, no putative PCP degradative enzymes were detected. This work constitutes the first toxicoproteomic study involving a Zygomycota fungus and the very first concerning the effect of PCP in a fungal proteome.
PID Serval
serval:BIB_1ECE5482ADE4
PMID
Date de création
2017-04-19T16:12:42.321Z
Date de création dans IRIS
2025-05-20T18:13:35Z