Titre
Altered peptide ligands trigger perforin- rather than Fas-dependent cell lysis
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Bachmann, M. F.
Auteure/Auteur
Ohteki, T.
Auteure/Auteur
Faienza, K. M.
Auteure/Auteur
Zakarian, A.
Auteure/Auteur
Kagi, D.
Auteure/Auteur
Speiser, D. E.
Auteure/Auteur
Ohashi, P. S.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0022-1767
Statut éditorial
Publié
Date de publication
1997-11
Volume
159
Numéro
9
Première page
4165
Dernière page/numéro d’article
70
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov 1
Research Support, Non-U.S. Gov't --- Old month value: Nov 1
Résumé
CTLs lyse Fas-expressing target cells by the concomitant action of a perforin- and a Fas-dependent mechanism. This study analyzed whether target cells pulsed with T cell antagonists and other altered peptide ligands (APLs) were susceptible selectively to only one of these two mechanisms. In vivo and in vitro activated T cells from transgenic mice expressing a TCR specific for lymphocytic choriomeningitis virus were used as effector cells. To distinguish between perforin- and Fas-dependent cytotoxicity, T cells from normal or perforin-deficient mice were used to lyse peptide-pulsed Fas-positive or Fas-negative target cells. In contrast to previous reports that have shown that APLs selectively induce the Fas-dependent pathway of cytotoxicity, our results demonstrate that target cells pulsed with T cell antagonists and other APLs are lysed predominantly by the perforin-dependent pathway. The contribution of Fas-mediated cytotoxicity was similar for the full agonist and the APLs. Thus, full agonists, partial agonists, and antagonists trigger similar and not distinct pathways of cytotoxicity.
Sujets
PID Serval
serval:BIB_6E509B10BF97
PMID
Date de création
2008-01-28T10:32:57.216Z
Date de création dans IRIS
2025-05-21T02:37:34Z