Titre
Antigenic specificity of the cytolytic T lymphocyte response to murine sarcoma virus-induced tumors. III. Characterization of cytolytic T lymphocyte clones specific for Moloney leukemia virus-associated cell surface antigens.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Weiss, A.
Auteure/Auteur
Brunner, K.T.
Auteure/Auteur
MacDonald, H.R.
Auteure/Auteur
Cerottini, J.C.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0022-1007
Statut éditorial
Publié
Date de publication
1980-11-01
Volume
152
Numéro
5
Première page
1210
Dernière page/numéro d’article
1225
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Résumé
Cytolytic T lymphocyte (CTL) clones specific for Moloney leukemia virus (MoLV)-derived tumor cells were generated by placing limiting numbers of C57BL/6 responder cells into mixed leukocyte-tumor cell microcultures. Under appropriate conditions (presence of stimulating tumor cells, accessory cells, and T cell growth factor), such cloned CTL could readily be expanded to provide large numbers of homogeneous, highly cytolytic CTL populations for further characterization. Using four target-cell types, three specificity patterns were observed: one reactive with the syngeneic MoLV-derived tumor only, one cross-reactive with an allogeneic MoLV-derived tumor, and one cross-reactive with normal allogeneic cells. Subclones derived from these three types of clones exhibited a high degree of stability in terms of lytic activity and specificity over a 4-mo period of observation. Three clones analyzed by flow cytofluorometry using monoclonal antibodies were all found to be of the Lyt-1+2+ phenotype. Furthermore, lysis of target cells by all of six clones tested was inhibited by anti-H-2Db (but not by anti-H-2Kb) monoclonal antibodies, demonstrating H-2Db-restriction at the clonal level.
PID Serval
serval:BIB_B137FD4BC144
PMID
Open Access
Oui
Date de création
2008-01-28T10:13:12.736Z
Date de création dans IRIS
2025-05-20T23:41:52Z
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Nom
6159445_BIB_B137FD4BC144.pdf
Version du manuscrit
published
Taille
845 KB
Format
Adobe PDF
PID Serval
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Somme de contrôle
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