Titre
Mini invasive skeletal muscle biopsy technique with a tri-axial end cut needle.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
European Review For Medical and Pharmacological Sciences
Auteur(s)
Invernizzi, M.
Auteure/Auteur
Rizzi, M.
Auteure/Auteur
Carda, S.
Auteure/Auteur
Cisari, C.
Auteure/Auteur
Molinari, C.
Auteure/Auteur
Renò, F.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
2284-0729
Statut éditorial
Publié
Date de publication
2015
Volume
19
Numéro
13
Première page
2446
Dernière page/numéro d’article
2451
Peer-reviewed
Oui
Langue
anglais
Résumé
OBJECTIVE: Skeletal Muscle Biopsy is a minor surgical procedure for the diagnosis of different neuromuscular pathological conditions and has recently gained popularity also in the research field of age-related muscular modifications and sarcopenia. Few studies focused on the application of mini-invasive muscular biopsy in both normal and pathological conditions. The aim of our study was to describe a mini invasive ultrasound-guided skeletal muscular biopsy technique in complete spinal cord injured (SCI) patients and healthy controls with a tri-axial end-cut needle.
PATIENTS AND METHODS: Skeletal muscle biopsies were collected from 6 chronic SCI patients and 3 healthy controls vastus lateralis muscle with a tri-axial end cut needle (Biopince© - Angiotech). Muscle samples were stained for ATPase to determine fibers composition, moreover, gene expression of cyclooxygenase-1 (COX-1) and prostaglandin E2 receptor has been analyzed by Real Time RT-PCR.
RESULTS: All the procedures were perfomed easily without failures and complications. Control tissue was macroscopically thicker than SCI one. Control specimen displayed an equal distribution of type I and type II fibers, while SCI sample displayed a prevalence of type II fibers SCI specimen displayed a significant reduction in COX-1 gene expression. This mini-invasive approach was easy, accurate and with low complication rate in performing skeletal muscle biopsy in both SCI patients and controls.
CONCLUSIONS: This technique could be useful in conditions in which the overall quantity of specimen required is small like for molecular biology analysis. For histological diagnostic purposes and/or conditions in which the original tissue is already pathologically modified, this technique should be integrated with more invasive techniques.
PATIENTS AND METHODS: Skeletal muscle biopsies were collected from 6 chronic SCI patients and 3 healthy controls vastus lateralis muscle with a tri-axial end cut needle (Biopince© - Angiotech). Muscle samples were stained for ATPase to determine fibers composition, moreover, gene expression of cyclooxygenase-1 (COX-1) and prostaglandin E2 receptor has been analyzed by Real Time RT-PCR.
RESULTS: All the procedures were perfomed easily without failures and complications. Control tissue was macroscopically thicker than SCI one. Control specimen displayed an equal distribution of type I and type II fibers, while SCI sample displayed a prevalence of type II fibers SCI specimen displayed a significant reduction in COX-1 gene expression. This mini-invasive approach was easy, accurate and with low complication rate in performing skeletal muscle biopsy in both SCI patients and controls.
CONCLUSIONS: This technique could be useful in conditions in which the overall quantity of specimen required is small like for molecular biology analysis. For histological diagnostic purposes and/or conditions in which the original tissue is already pathologically modified, this technique should be integrated with more invasive techniques.
PID Serval
serval:BIB_08825D4BF422
PMID
Date de création
2015-08-28T16:45:52.782Z
Date de création dans IRIS
2025-05-20T19:59:06Z
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Nom
2446-2451.pdf
Version du manuscrit
published
Licence
https://iris.unil.ch/disclaimer
Taille
167.77 KB
Format
Adobe PDF
PID Serval
serval:BIB_08825D4BF422.P001
URN
urn:nbn:ch:serval-BIB_08825D4BF4220
Somme de contrôle
(MD5):b1910f350057d5767f178430ade6188f