Titre
Comprehensive map of ribosomal 2'-O-methylation and C/D box snoRNAs in Drosophila melanogaster.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Sklias, A.
Auteure/Auteur
Cruciani, S.
Auteure/Auteur
Marchand, V.
Auteure/Auteur
Spagnuolo, M.
Auteure/Auteur
Lavergne, G.
Auteure/Auteur
Bourguignon, V.
Auteure/Auteur
Brambilla, A.
Auteure/Auteur
Dreos, R.
Auteure/Auteur
Marygold, S.J.
Auteure/Auteur
Novoa, E.M.
Auteure/Auteur
Motorin, Y.
Auteure/Auteur
Roignant, J.Y.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1362-4962
Statut éditorial
Publié
Date de publication
2024-04-12
Volume
52
Numéro
6
Première page
2848
Dernière page/numéro d’article
2864
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Résumé
During their maturation, ribosomal RNAs (rRNAs) are decorated by hundreds of chemical modifications that participate in proper folding of rRNA secondary structures and therefore in ribosomal function. Along with pseudouridine, methylation of the 2'-hydroxyl ribose moiety (Nm) is the most abundant modification of rRNAs. The majority of Nm modifications in eukaryotes are placed by Fibrillarin, a conserved methyltransferase belonging to a ribonucleoprotein complex guided by C/D box small nucleolar RNAs (C/D box snoRNAs). These modifications impact interactions between rRNAs, tRNAs and mRNAs, and some are known to fine tune translation rates and efficiency. In this study, we built the first comprehensive map of Nm sites in Drosophila melanogaster rRNAs using two complementary approaches (RiboMethSeq and Nanopore direct RNA sequencing) and identified their corresponding C/D box snoRNAs by whole-transcriptome sequencing. We de novo identified 61 Nm sites, from which 55 are supported by both sequencing methods, we validated the expression of 106 C/D box snoRNAs and we predicted new or alternative rRNA Nm targets for 31 of them. Comparison of methylation level upon different stresses show only slight but specific variations, indicating that this modification is relatively stable in D. melanogaster. This study paves the way to investigate the impact of snoRNA-mediated 2'-O-methylation on translation and proteostasis in a whole organism.
PID Serval
serval:BIB_6F7BAC3B84B8
PMID
Open Access
Oui
Date de création
2024-03-01T10:21:24.174Z
Date de création dans IRIS
2025-05-20T21:27:02Z
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Nom
38416577.pdf
Version du manuscrit
published
Licence
https://creativecommons.org/licenses/by-nc/4.0
Taille
1.95 MB
Format
Adobe PDF
PID Serval
serval:BIB_6F7BAC3B84B8.P001
URN
urn:nbn:ch:serval-BIB_6F7BAC3B84B86
Somme de contrôle
(MD5):bed9accd8ca42887d715922b0ed41b98