Titre
High-dimensional immune phenotyping of blood cells by mass cytometry in patients infected with hepatitis C virus.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Herderschee, J.
Auteure/Auteur
Heinonen, T.
Auteure/Auteur
Fenwick, C.
Auteure/Auteur
Schrijver, I.T.
Auteure/Auteur
Ohmiti, K.
Auteure/Auteur
Moradpour, D.
Auteure/Auteur
Cavassini, M.
Auteure/Auteur
Pantaleo, G.
Auteure/Auteur
Roger, T.
Auteure/Auteur
Calandra, T.
Auteure/Auteur
Contributrices/contributeurs
Aebi-Popp, K.
Anagnostopoulos, A.
Battegay, M.
Bernasconi, E.
Böni, J.
Braun, D.L.
Bucher, H.C.
Calmy, A.
Cavassini, M.
Ciuffi, A.
Dollenmaier, G.
Egger, M.
Elzi, L.
Fehr, J.
Fellay, J.
Furrer, H.
Fux, C.A.
Günthard, H.F.
Haerry, D.
Hasse, B.
Hirsch, H.H.
Hoffmann, M.
Hösli, I.
Huber, M.
Kahlert, C.R.
Kaiser, L.
Keiser, O.
Klimkait, T.
Kouyos, R.D.
Kovari, H.
Ledergerber, B.
Martinetti, G.
Martinez de Tejada, B.
Marzolini, C.
Metzner, K.J.
Müller, N.
Nicca, D.
Paioni, P.
Pantaleo, G.
Perreau, M.
Rauch, A.
Rudin, C.
Scherrer, A.U.
Schmid, P.
Speck, R.
Stöckle, M.
Tarr, P.
Trkola, A.
Vernazza, P.
Wandeler, G.
Weber, R.
Yerly, S.
Groupes de travail
Swiss HIV Cohort Study
Liens vers les unités
ISSN
1469-0691
Statut éditorial
Publié
Date de publication
2022-04
Volume
28
Numéro
4
Première page
611.e1
Dernière page/numéro d’article
611.e7
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Résumé
Chronic hepatitis C virus (HCV) infection affects the immune system. Whether elimination of HCV with direct-acting antivirals (DAA) restores immunity is unclear. We used mass cytometry to get a broad and in-depth assessment of blood cell populations of patients with chronic HCV before and after DAA therapy.
Before and 12 weeks after sustained virological response (SVR12) to DAA therapy, 22 cell populations were analysed by mass cytometry in blood collected from ten healthy control individuals and 20 HCV-infected patients with (ten patients) or without (ten patients) human immunodeficiency virus (HIV) infection.
HCV infection altered the frequency of 14/22 (64%) blood cell populations. At baseline, the frequencies (median, interquartile range (IQR); control, HCV, HCV/HIV) of intermediate monocytes (1.2, IQR 0.47-1.46; 1.76, IQR 0.83-2.66; 0.78, IQR 0.28-1.77), non-classical monocytes (1.11, IQR 0.49-1.26; 0.9, IQR 0.18-0.99; 0.54, IQR 0.28-1.77), conventional dendritic cells type 2 (0.55, IQR 0.35-0.59; 0.31, IQR 0.16-0.38; 0.19, IQR 0.11-0.36) and CD56 <sup>dim</sup> natural killer cells (8.08, IQR 5.34-9.79; 4.72, IQR 2.59-6.05) 3.61, IQR 2.98-5.07) were reduced by 35% to 65%, particularly in HCV/HIV co-infected patients. In contrast, activated double-negative T cells (0.07, IQR 0.06-0.10; 0.10, IQR 0.09-0.19; 0.19, IQR 0.12-0.25), activated CD4 T cells (0.28, IQR 0.21-0.36; 0.56, IQR 0.33-0.77; 0.40, IQR 0.22-0.53) and activated CD8 T cells (0.23, IQR 0.14-0.42; 0.74, IQR 0.30-1.65; 0.80, IQR 0.58-1.16) were increased 1.4 to 3.5 times. Upon stimulation with Toll-like receptor ligands, the expression of cytokines was up-regulated in 7/9 (78%) and 17/19 (89%) of the conditions in HCV- and HCV/HIV-infected patients, respectively. Most alterations persisted at SVR12.
Chronic HCV and HCV/HIV infections induce profound and durable perturbations of innate and adaptive immune homeostasis.
Before and 12 weeks after sustained virological response (SVR12) to DAA therapy, 22 cell populations were analysed by mass cytometry in blood collected from ten healthy control individuals and 20 HCV-infected patients with (ten patients) or without (ten patients) human immunodeficiency virus (HIV) infection.
HCV infection altered the frequency of 14/22 (64%) blood cell populations. At baseline, the frequencies (median, interquartile range (IQR); control, HCV, HCV/HIV) of intermediate monocytes (1.2, IQR 0.47-1.46; 1.76, IQR 0.83-2.66; 0.78, IQR 0.28-1.77), non-classical monocytes (1.11, IQR 0.49-1.26; 0.9, IQR 0.18-0.99; 0.54, IQR 0.28-1.77), conventional dendritic cells type 2 (0.55, IQR 0.35-0.59; 0.31, IQR 0.16-0.38; 0.19, IQR 0.11-0.36) and CD56 <sup>dim</sup> natural killer cells (8.08, IQR 5.34-9.79; 4.72, IQR 2.59-6.05) 3.61, IQR 2.98-5.07) were reduced by 35% to 65%, particularly in HCV/HIV co-infected patients. In contrast, activated double-negative T cells (0.07, IQR 0.06-0.10; 0.10, IQR 0.09-0.19; 0.19, IQR 0.12-0.25), activated CD4 T cells (0.28, IQR 0.21-0.36; 0.56, IQR 0.33-0.77; 0.40, IQR 0.22-0.53) and activated CD8 T cells (0.23, IQR 0.14-0.42; 0.74, IQR 0.30-1.65; 0.80, IQR 0.58-1.16) were increased 1.4 to 3.5 times. Upon stimulation with Toll-like receptor ligands, the expression of cytokines was up-regulated in 7/9 (78%) and 17/19 (89%) of the conditions in HCV- and HCV/HIV-infected patients, respectively. Most alterations persisted at SVR12.
Chronic HCV and HCV/HIV infections induce profound and durable perturbations of innate and adaptive immune homeostasis.
Sujets
PID Serval
serval:BIB_300AF1BADC64
PMID
Open Access
Oui
Date de création
2021-10-04T11:55:11.849Z
Date de création dans IRIS
2025-05-20T16:30:15Z
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Nom
CMI_Herderschee.pdf
Version du manuscrit
postprint
Taille
2.7 MB
Format
Adobe PDF
PID Serval
serval:BIB_300AF1BADC64.P001
URN
urn:nbn:ch:serval-BIB_300AF1BADC643
Somme de contrôle
(MD5):71dfa246ec0bbf4ca384bee97a278222