Titre
A natural structural variant of the mouse TCR beta-chain displays intrinsic receptor function and antigen specificity.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Aublin, A.
Auteure/Auteur
Ciofani, M.
Auteure/Auteur
Willkomm, N.
Auteure/Auteur
Hamrouni, A.
Auteure/Auteur
Szymczak-Workman, A.L.
Auteure/Auteur
Takahashi, T.
Auteure/Auteur
Sandjeu, Y.
Auteure/Auteur
Guillaume, P.
Auteure/Auteur
Vignali, D.A.
Auteure/Auteur
Michielin, O.
Auteure/Auteur
Zúñiga-Pflücker, J.C.
Auteure/Auteur
Maryanski, J.L.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0022-1767
Statut éditorial
Publié
Date de publication
2006
Volume
177
Numéro
12
Première page
8587
Dernière page/numéro d’article
8594
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Résumé
The Cbeta0 alternate cassette exon is located between the Jbeta1 and Cbeta1 genes in the mouse TCR beta-locus. In T cells with a VDJbeta1 rearrangement, the Cbeta0 exon may be included in TCRbeta transcripts (herein called TCRbeta-Cbeta0 transcripts), potentially inserting an additional 24 aa between the V and C domains of the TCR beta-chain. These TCRbeta splice isoforms may be differentially regulated after Ag activation, because we detected TCRbeta-Cbeta0 transcripts in a high proportion (>60%) of immature and mature T cells having VDJbeta1 rearrangements but found a substantially reduced frequency (<35%) of TCRbeta-Cbeta0 expression among CD8 T cells selected by Ag in vivo. To study the potential activity of the TCRbeta-Cbeta0 splice variant, we cloned full-length TCR cDNAs by single-cell RT-PCR into retroviral expression vectors. We found that the TCRbeta-Cbeta0 splice isoform can function during an early stage of T cell development normally dependent on TCR beta-chain expression. We also demonstrate that T hybridoma-derived cells expressing a TCRbeta-Cbeta0 isoform together with the clonally associated TCR alpha-chain recognize the same cognate peptide-MHC ligand as the corresponding normal alphabetaTCR. This maintenance of receptor function and specificity upon insertion of the Cbeta0 peptide cassette signifies a remarkable adaptability for the TCR beta-chain, and our findings open the possibility that this splice isoform may function in vivo.
PID Serval
serval:BIB_A35076FF376B
PMID
Date de création
2008-01-28T10:22:46.777Z
Date de création dans IRIS
2025-05-20T22:06:04Z