Titre
Translation is required for miRNA-dependent decay of endogenous transcripts.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Biasini, A.
Auteure/Auteur
Abdulkarim, B.
Auteure/Auteur
de Pretis, S.
Auteure/Auteur
Tan, J.Y.
Auteure/Auteur
Arora, R.
Auteure/Auteur
Wischnewski, H.
Auteure/Auteur
Dreos, R.
Auteure/Auteur
Pelizzola, M.
Auteure/Auteur
Ciaudo, C.
Auteure/Auteur
Marques, A.C.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1460-2075
Statut éditorial
Publié
Date de publication
2021-02-01
Volume
40
Numéro
3
Première page
e104569
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Résumé
Post-transcriptional repression of gene expression by miRNAs occurs through transcript destabilization or translation inhibition. mRNA decay is known to account for most miRNA-dependent repression. However, because transcript decay occurs co-translationally, whether target translation is a requirement for miRNA-dependent transcript destabilization remains unknown. To decouple these two molecular processes, we used cytosolic long noncoding RNAs (lncRNAs) as models for endogenous transcripts that are not translated. We show that, despite interacting with the miRNA-loaded RNA-induced silencing complex, the steady-state abundance and decay rates of these transcripts are minimally affected by miRNA loss. To further validate the apparent requirement of translation for miRNA-dependent decay, we fused two lncRNA candidates to the 3'-end of a protein-coding gene reporter and found this results in their miRNA-dependent destabilization. Further analysis revealed that the few natural lncRNAs whose levels are regulated by miRNAs in mESCs tend to associate with translating ribosomes, and possibly represent misannotated micropeptides, further substantiating the necessity of target translation for miRNA-dependent transcript decay. In summary, our analyses suggest that translation is required for miRNA-dependent transcript destabilization, and demonstrate that the levels of coding and noncoding transcripts are differently affected by miRNAs.
Sujets
PID Serval
serval:BIB_1E9949CC6BEE
PMID
Open Access
Oui
Date de création
2020-12-22T10:14:37.752Z
Date de création dans IRIS
2025-05-20T17:23:06Z
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Nom
33300180_BIB_1E9949CC6BEE.pdf
Version du manuscrit
published
Licence
https://creativecommons.org/licenses/by/4.0
Taille
891.35 KB
Format
Adobe PDF
PID Serval
serval:BIB_1E9949CC6BEE.P001
URN
urn:nbn:ch:serval-BIB_1E9949CC6BEE5
Somme de contrôle
(MD5):184c470d932e3bb68ef82300979b8b0c