Titre
Na(+)-K(+)-ATPase gene expression during in vitro development of rat fetal forebrain.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Auteur(s)
Corthésy-Theulaz, I.
Auteure/Auteur
Mérillat, A.M.
Auteure/Auteur
Honegger, P.
Auteure/Auteur
Rossier, B.C.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0363-6143
Statut éditorial
Publié
Date de publication
1990
Volume
258
Numéro
6 Pt 1
Première page
C1062
Dernière page/numéro d’article
C1069
Langue
anglais
Résumé
The existence of at least three isoforms of Na(+)-K(+)-ATPase in adult brain tissues [alpha 1, kidney type; alpha 2 [or alpha(+)]; alpha 3] suggests that these genes might be regulated in a cell-specific and time-dependent manner during development. We have studied this question in serum-free aggregating cell cultures of mechanically dissociated rat fetal telencephalon. At the protein level, the relative rate of synthesis of the pool of alpha 1-, alpha 2-, and alpha 3-subunits increased approximately twofold over 15 days of culture, leading to a marked increase in the immunochemical pool of alpha-subunits as measured by a panspecific polyclonal antibody. Concomitantly, Na(+)-K(+)-ATPase enzyme-specific activity increased three- (lower forebrain) to sixfold (upper forebrain). The transcripts of all three alpha-isoforms and beta-subunit were detected in vitro in similar proportion to the level observed in vivo. alpha 3-mRNA (3.7 kb) was more abundant than alpha 1 (3.7 kb) or alpha 2 (5.3 and 3.4 kb). Cytosine arabinoside (0.4 microM) and cholera toxin (0.1 microM) were used to selectively eliminate glial cells or neurons, respectively. It was found that alpha 2-mRNA is predominantly transcribed in glial cell cultures, whereas alpha 3- and beta 1-mRNA (2.7, 2.3, and 1.8 kb) are predominant in neuronal cultures.
Sujets
PID Serval
serval:BIB_B9EB77E42D0A
PMID
Date de création
2008-01-24T12:00:24.822Z
Date de création dans IRIS
2025-05-20T21:41:46Z