Titre
Ploidy of Bacillus subtilis exfusants: the haploid nature of cells forming colonies with biparental or prototrophic phenotypes
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Journal of General Microbiology
Auteur(s)
Hauser, P. M.
Auteure/Auteur
Karamata, D.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0022-1287
Statut éditorial
Publié
Date de publication
1992-06
Volume
138
Numéro
6
Première page
1077
Dernière page/numéro d’article
88
Notes
Journal Article --- Old month value: Jun
Résumé
To investigate the relationship between DNA content and cell volume, we have attempted to repeat the construction of stable Bacillus subtilis diploid cells through protoplast fusion. Colonies with a biparental phenotype and those with a prototrophic phenotype were identified among exfusants of a cross between two polyauxotrophic strains. The ploidy of cells constituting such colonies was assessed by protoplast self-fusion, determination of the DNA to dry weight ratio of exponentially growing cells, and by quantitative DNA-DNA hybridization. Within the precision of these methods, all colonies were found to consist of haploid cells. A previously described non-complementing diploid was also found to be haploid. Therefore, the genetic evidence in favour of diploidy, based on continuing segregation of cells with a parental or recombinant phenotype, cannot be accounted for except by the maintenance of such cells as a minority population in mixed colonies through cross-feeding. Reconstruction experiments with mixtures of whole parental cells confirm that biparental colonies are indeed mixed colonies which arise either by sticking of parental cells or through coincidence, i.e. their plating within a distance of about 0.4 mm. The previously reported experimental results can be accounted for in the light of our results.
Sujets
PID Serval
serval:BIB_CE8524541FAE
PMID
Date de création
2008-01-25T13:30:08.835Z
Date de création dans IRIS
2025-05-21T02:25:40Z