Titre
The use of 68Ga-EDTA PET allows detecting progressive decline of renal function in rats.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Auteur(s)
Fontana, A.O.
Auteure/Auteur
Gonzalez Melo, M.
Auteure/Auteur
Allenbach, G.
Auteure/Auteur
Georgantas, C.
Auteure/Auteur
Wang, R.
Auteure/Auteur
Braissant, O.
Auteure/Auteur
Barbey, F.
Auteure/Auteur
Prior, J.O.
Auteure/Auteur
Ballhausen, D.
Auteure/Auteur
Viertl, D.
Auteure/Auteur
Liens vers les unités
ISSN
2160-8407
Statut éditorial
Publié
Date de publication
2021
Volume
11
Numéro
6
Première page
519
Dernière page/numéro d’article
528
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Publication Status: epublish
Résumé
Evaluation of glomerular filtration rate is very important in both preclinical and clinical setting, especially in the context of chronic kidney disease. It is typically performed using <sup>51</sup> Cr-EDTA or by imaging with <sup>123</sup> I-Hippuran scintigraphy, which has a significantly lower resolution and sensitivity as compared to PET. <sup>68</sup> Ga-EDTA represents a valid alternative due to its quick availability using a <sup>68</sup> Ge/ <sup>68</sup> Ga generator, while PET/CT enables both imaging of renal function and accurate quantitation of clearance of activity from both plasma and urine. Therefore, we aimed at investigating the use of <sup>68</sup> Ga-EDTA as a preclinical tracer for determining renal function in a knock-in rat model known to present progressive decline of renal function.
<sup>68</sup> Ga-EDTA was injected in 23 rats, either wild type (n=10) or knock-in (n=13). By applying a unidirectional, two-compartment model and Rutland-Patlak Plot linear regression analysis, split renal function was determined from the age of 6 weeks to 12 months.
Glomerular filtration ranged from 0.025±0.01 ml/min at 6 weeks to 0.049±0.05 ml/min at 6 months in wild type rats. Glomerular filtration was significantly lower in knock-in rats at 6 and 12 months (P<0.01). No significant difference was observed in renal volumes between knock-in and wild type animals, based on imaging-derived volume calculations.
<sup>68</sup> Ga-EDTA turned out to be a very promising PET/CT tracer for the evaluation of split renal function. This method allowed detection of progressive renal impairment in a knock-in rat model. Additional validation in a human cohort is warranted to further assess clinical utility in both, healthy individuals and patients with renal impairment.
<sup>68</sup> Ga-EDTA was injected in 23 rats, either wild type (n=10) or knock-in (n=13). By applying a unidirectional, two-compartment model and Rutland-Patlak Plot linear regression analysis, split renal function was determined from the age of 6 weeks to 12 months.
Glomerular filtration ranged from 0.025±0.01 ml/min at 6 weeks to 0.049±0.05 ml/min at 6 months in wild type rats. Glomerular filtration was significantly lower in knock-in rats at 6 and 12 months (P<0.01). No significant difference was observed in renal volumes between knock-in and wild type animals, based on imaging-derived volume calculations.
<sup>68</sup> Ga-EDTA turned out to be a very promising PET/CT tracer for the evaluation of split renal function. This method allowed detection of progressive renal impairment in a knock-in rat model. Additional validation in a human cohort is warranted to further assess clinical utility in both, healthy individuals and patients with renal impairment.
PID Serval
serval:BIB_A42CB7293042
PMID
Date de création
2022-01-17T10:15:07.221Z
Date de création dans IRIS
2025-05-20T21:11:33Z
Fichier(s)![Vignette d'image]()
En cours de chargement...
Nom
2021-AJNMMI Fontana.pdf
Version du manuscrit
preprint
Taille
2.41 MB
Format
Adobe PDF
PID Serval
serval:BIB_A42CB7293042.P001
Somme de contrôle
(MD5):229a157c59abf9cf0449569adc10e525