Titre
Circulating microRNAs as biomarkers for detection of autologous blood transfusion.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Leuenberger, N.
Auteure/Auteur
Schumacher, Y.O.
Auteure/Auteur
Pradervand, S.
Auteure/Auteur
Sander, T.
Auteure/Auteur
Saugy, M.
Auteure/Auteur
Pottgiesser, T.
Auteure/Auteur
Liens vers les personnes
ISSN
1932-6203
Statut éditorial
Publié
Date de publication
2013
Volume
8
Numéro
6
Première page
e66309
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: epublish
Résumé
MicroRNAs (miRNAs) are small non-coding RNAs that regulate various biological processes. Cell-free miRNAs measured in blood plasma have emerged as specific and sensitive markers of physiological processes and disease. In this study, we investigated whether circulating miRNAs can serve as biomarkers for the detection of autologous blood transfusion, a major doping technique that is still undetectable. Plasma miRNA levels were analyzed using high-throughput quantitative real-time PCR. Plasma samples were obtained before and at several time points after autologous blood transfusion (blood bag storage time 42 days) in 10 healthy subjects and 10 controls without transfusion. Other serum markers of erythropoiesis were determined in the same samples. Our results revealed a distinct change in the pattern of circulating miRNAs. Ten miRNAs were upregulated in transfusion samples compared with control samples. Among these, miR-30b, miR-30c, and miR-26b increased significantly and showed a 3.9-, 4.0-, and 3.0-fold change, respectively. The origin of these miRNAs was related to pulmonary and liver tissues. Erythropoietin (EPO) concentration decreased after blood reinfusion. A combination of miRNAs and EPO measurement in a mathematical model enhanced the efficiency of autologous transfusion detection through miRNA analysis. Therefore, our results lay the foundation for the development of miRNAs as novel blood-based biomarkers to detect autologous transfusion.
PID Serval
serval:BIB_67101E35A815
PMID
Open Access
Oui
Date de création
2013-09-06T12:27:40.315Z
Date de création dans IRIS
2025-05-21T01:59:15Z
Fichier(s)![Vignette d'image]()
En cours de chargement...
Nom
BIB_67101E35A815.P001.pdf
Version du manuscrit
published
Taille
641.53 KB
Format
Adobe PDF
PID Serval
serval:BIB_67101E35A815.P001
URN
urn:nbn:ch:serval-BIB_67101E35A8151
Somme de contrôle
(MD5):2393d026af7ca2d03e53315fa6ed1b8a