Titre
Ethylene glycol and amino acid derivatives of 5-aminolevulinic acid as new photosensitizing precursors of protoporphyrin IX in cells
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Berger, Y.
Auteure/Auteur
Greppi, A.
Auteure/Auteur
Siri, O.
Auteure/Auteur
Neier, R.
Auteure/Auteur
Juillerat-Jeanneret, L.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0022-2623
Statut éditorial
Publié
Date de publication
2000
Volume
43
Numéro
25
Première page
4738
Dernière page/numéro d’article
4746
Langue
anglais
Notes
PT - Journal Article PT - Research Support, Non-U.S. Gov't
Résumé
Protoporphyrin IX (PpIX) is used as a photosensitizing agent in photodynamic detection and therapy (PDT) of cancer and is synthesized intracellularly from aminolevulinic acid (ALA) precursors. To evaluate means to specifically target ALA derivatives to defined cells, we have synthesized and characterized ethylene glycol esters and amino acid pseudodipeptide derivatives of ALA as potential specific substrates for cellular esterases and aminopeptidases, respectively. The PpIX formation induced by these products was investigated using cultures of human and rat cell lines of carcinoma and endothelial origins. The cytotoxicity of these compounds in the absence of light was also controlled. The results have shown that ethylenglycol esters can induce high levels of PpIX and are useful at concentrations below their cytotoxicity threshold. From the ALA-amino acid derivatives which were evaluated, the highest PpIX production was obtained using ALA derivatives of neutral amino acids, as compared to acidic or basic amino acids
Sujets
PID Serval
serval:BIB_0B2B04E9FA52
PMID
Date de création
2008-01-29T17:33:12.335Z
Date de création dans IRIS
2025-05-20T18:38:19Z
Fichier(s)![Vignette d'image]()
En cours de chargement...
Nom
11123982.pdf
Version du manuscrit
published
Licence
https://iris.unil.ch/disclaimer
Taille
1.8 MB
Format
Adobe PDF
PID Serval
serval:BIB_0B2B04E9FA52.P001
URN
urn:nbn:ch:serval-BIB_0B2B04E9FA521
Somme de contrôle
(MD5):a3f12c9bceb68aed98a3ae9222d60fb7