Titre
Immature CD4- CD8+ murine thymocytes.
Type
article
Institution
Externe
Périodique
Auteur(s)
Shortman, K.
Auteure/Auteur
Wilson, A.
Auteure/Auteur
Egerton, M.
Auteure/Auteur
Pearse, M.
Auteure/Auteur
Scollay, R.
Auteure/Auteur
Liens vers les personnes
ISSN
0008-8749
Statut éditorial
Publié
Date de publication
1988
Volume
113
Numéro
2
Première page
462
Dernière page/numéro d’article
479
Langue
anglais
Résumé
Mature thymocytes are usually defined and separated from other less mature thymocytes on the basis of their mutually exclusive expression of either CD4 or CD8. However, such murine "single positives" include a subpopulation of immature cells with properties resembling CD4- CD8- thymocytes or CD4+ CD8+ cortical blasts. Most of these immature single positives are CD4- CD8+, some expressing relatively low levels of CD8. They are large, dividing cortisone-sensitive cells found in the outer cortex. They express high levels of the heat-stable antigen (recognized by the monoclonals M1/69, B2A2, and J11d) but they are MEL-14-. The absence of detectable surface CD3, the absence of alpha-chain messenger RNA, and the predominance of the truncated form of the beta-chain messenger RNA all indicate that they do not express the T-cell antigen-receptor complex. Strategies for eliminating such immature cells from preparations of mature thymocytes are given, and their developmental significance is discussed.
Sujets
PID Serval
serval:BIB_555F2598AD6F
PMID
Date de création
2013-04-10T11:51:24.655Z
Date de création dans IRIS
2025-05-20T16:30:28Z