Titre
PKA antagonizes CLASP-dependent microtubule stabilization to re-localize Pom1 and buffer cell size upon glucose limitation.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Kelkar, M.
Auteure/Auteur
Martin, S.G.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
2041-1723
Statut éditorial
Publié
Date de publication
2015
Volume
6
Première page
8445
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Video-Audio Media
Publication Status: epublish
Publication Status: epublish
Résumé
Cells couple growth with division and regulate size in response to nutrient availability. In rod-shaped fission yeast, cell-size control occurs at mitotic commitment. An important regulator is the DYRK-family kinase Pom1, which forms gradients from cell poles and inhibits the mitotic activator Cdr2, itself localized at the medial cortex. Where and when Pom1 modulates Cdr2 activity is unclear as Pom1 medial cortical levels remain constant during cell elongation. Here we show that Pom1 re-localizes to cell sides upon environmental glucose limitation, where it strongly delays mitosis. This re-localization is caused by severe microtubule destabilization upon glucose starvation, with microtubules undergoing catastrophe and depositing the Pom1 gradient nucleator Tea4 at cell sides. Microtubule destabilization requires PKA/Pka1 activity, which negatively regulates the microtubule rescue factor CLASP/Cls1/Peg1, reducing CLASP's ability to stabilize microtubules. Thus, PKA signalling tunes CLASP's activity to promote Pom1 cell side localization and buffer cell size upon glucose starvation.
Sujets
PID Serval
serval:BIB_8D3C480D64EF
PMID
Open Access
Oui
Date de création
2015-12-10T21:54:20.574Z
Date de création dans IRIS
2025-05-21T03:45:40Z
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Nom
BIB_8D3C480D64EF.P001.pdf
Version du manuscrit
published
Taille
7.39 MB
Format
Adobe PDF
PID Serval
serval:BIB_8D3C480D64EF.P001
URN
urn:nbn:ch:serval-BIB_8D3C480D64EF8
Somme de contrôle
(MD5):3f169fc3f1ebabe297b9591cd419cf90