Titre
Performance evaluation of the Becton Dickinson Kiestra™ IdentifA/SusceptA.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Jacot, D.
Auteure/Auteur
Sarton-Lohéac, G.
Auteure/Auteur
Coste, A.T.
Auteure/Auteur
Bertelli, C.
Auteure/Auteur
Greub, G.
Auteure/Auteur
Prod'hom, G.
Auteure/Auteur
Croxatto, A.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1469-0691
Statut éditorial
Publié
Date de publication
2021-08
Volume
27
Numéro
8
Première page
1167.e9
Dernière page/numéro d’article
1167.e17
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Résumé
New automated modules are required to provide fully automated solutions in diagnostic microbiology laboratories. We evaluated the performance of a Becton Dickinson Kiestra™ IdentifA/SusceptA prototype for MALDI-TOF identification (ID) and Phoenix™ antibiotic susceptibility testing (AST).
The performance of the IdentifA/SusceptA coupled prototype was compared with manual processing for MALDI-TOF ID on 1302 clinical microbial isolates or ATCC strains and for Phoenix™ M50 AST on 484 strains, representing 61 species.
Overall, the IdentifA exhibited similar ID performances than manual spotting. Higher performances were observed for Gram-negative bacteria with an ID at the species level (score >2) of 96.5% (369/382) and 86.9% (334/384), respectively. A significantly better performance was observed with the IdentifA (95.2%, 81/85) compared with manual spotting (75.2%, 64/85) from colonies on MacConkey agar. Contrariwise, the IdentifA exhibited lower ID performances at the species level than manual processing for streptococci (76.1%, 96/126 compared with 92%, 115/125), coagulase-negative staphylococci (73.3%, 44/60 compared with 90%, 54/60) and yeasts (41.3%, 19/46 compared with 78.2%, 36/46). Staphylococcus aureus and enterococci were similarly identified by the two approaches, with ID rates of 92% (65/70) for the IdentifA and 92.7%, (64/69) for manual processing and 94.8%, (55/58) for the IdentifA and 98.2%, (57/58) for manual processing, respectively. The SusceptA exhibited an AST overall essential agreement of 98.82% (6863/6945), a category agreement of 98.86% (6866/6945), 1.05% (6/570) very major errors, 0.16% (10/6290) major errors, and 0.91% (63/6945) minor errors compared to the reference AST.
Overall, the automated IdentifA/SusceptA exhibited high ID and AST performances.
The performance of the IdentifA/SusceptA coupled prototype was compared with manual processing for MALDI-TOF ID on 1302 clinical microbial isolates or ATCC strains and for Phoenix™ M50 AST on 484 strains, representing 61 species.
Overall, the IdentifA exhibited similar ID performances than manual spotting. Higher performances were observed for Gram-negative bacteria with an ID at the species level (score >2) of 96.5% (369/382) and 86.9% (334/384), respectively. A significantly better performance was observed with the IdentifA (95.2%, 81/85) compared with manual spotting (75.2%, 64/85) from colonies on MacConkey agar. Contrariwise, the IdentifA exhibited lower ID performances at the species level than manual processing for streptococci (76.1%, 96/126 compared with 92%, 115/125), coagulase-negative staphylococci (73.3%, 44/60 compared with 90%, 54/60) and yeasts (41.3%, 19/46 compared with 78.2%, 36/46). Staphylococcus aureus and enterococci were similarly identified by the two approaches, with ID rates of 92% (65/70) for the IdentifA and 92.7%, (64/69) for manual processing and 94.8%, (55/58) for the IdentifA and 98.2%, (57/58) for manual processing, respectively. The SusceptA exhibited an AST overall essential agreement of 98.82% (6863/6945), a category agreement of 98.86% (6866/6945), 1.05% (6/570) very major errors, 0.16% (10/6290) major errors, and 0.91% (63/6945) minor errors compared to the reference AST.
Overall, the automated IdentifA/SusceptA exhibited high ID and AST performances.
PID Serval
serval:BIB_85C11972FFBD
PMID
Open Access
Oui
Date de création
2020-10-26T06:34:50.032Z
Date de création dans IRIS
2025-05-21T00:35:47Z
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Nom
1-s2.0-S1198743X20306042-main.pdf
Version du manuscrit
preprint
Licence
https://creativecommons.org/licenses/by-nc-nd/4.0
Taille
456.6 KB
Format
Adobe PDF
PID Serval
serval:BIB_85C11972FFBD.P001
URN
urn:nbn:ch:serval-BIB_85C11972FFBD0
Somme de contrôle
(MD5):dddff88501c15c6019039b176a1a8a6a