Titre
Leukocyte's Hif-1 expression and training-induced erythropoietic response in swimmers
Type
article
Institution
Externe
Périodique
Auteur(s)
Mounier, R.
Auteure/Auteur
Pialoux, V.
Auteure/Auteur
Cayre, A.
Auteure/Auteur
Schmitt, L.
Auteure/Auteur
Richalet, J.P.
Auteure/Auteur
Robach, P.
Auteure/Auteur
Lasne, F.
Auteure/Auteur
Roels, B.
Auteure/Auteur
Millet, G.
Auteure/Auteur
Coudert, J.
Auteure/Auteur
Clottes, E.
Auteure/Auteur
Fellmann, N.
Auteure/Auteur
Liens vers les personnes
ISSN
0195-9131
Statut éditorial
Publié
Date de publication
2006-08
Volume
38
Numéro
8
Première page
1410
Dernière page/numéro d’article
1417
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Résumé
PURPOSE: Altitude training is popular among athletes to augment oxygen delivery capabilities to tissues and to improve physical performance. Hypoxia inducible factor-1 (HIF-1) controls the expression of several genes' encoding involved in physiological responses towards reduced oxygen availability, in particular by increasing serum erythropoietin (EPO). It may be involved in the individual variability for erythropoietic markers and/or sea-level performance of athletes using altitude during their training. Therefore, we investigated whether, before training, evolutions of hif-1alpha and ahif (HIF-1alpha natural antisense) transcript amounts and HIF-1alpha protein quantities in leukocytes measured during an acute hypoxia normobaric test (3 h at 3000 m at rest) could allow to predict poor and good responders for hematological markers after a "living high-training low" protocol. METHODS: Eighteen elite swimmers were divided into two groups that followed a 13-d training program: "living low-training low" (1200 m) (LL) or "living high (2500-3000 m)-training low (1200 m)" (LH). RESULTS: During the initial hypoxia test, a strong interindividual variability in the amounts of HIF-1alpha mRNA, aHIF transcript, and HIF-1alpha protein was observed in athlete leukocytes (after vs before): -82%/+396%, -100%/+229%, and -100%/+633%, respectively. After the test, serum erythropoietin concentration was increased (11.2 +/- 0.8 vs 9.8 +/- 0.8 IU.L(-1); +18%, P = 0.01). After the training protocol, total red cell volume (+7.6%, P = 0.04) and circulating hemoglobin amount (48.8 +/- 2.8 vs 45.5 +/- 3.0 mmol; i.e., +7.9%, P = 0.02) were significantly augmented in LH. CONCLUSION: We conclude that hif-1alpha gene expression quantification in leukocytes after a 3-h hypoxia test performed before training does not predict poor and good responder athletes to "living high-training low" model.
Sujets
PID Serval
serval:BIB_E9612C766101
PMID
Date de création
2008-09-25T07:01:10.250Z
Date de création dans IRIS
2025-05-21T07:20:56Z