Titre
Microarray and RNAi Analysis of P450s in Anopheles gambiae Male and Female Steroidogenic Tissues: CYP307A1 Is Required for Ecdysteroid Synthesis.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Pondeville, E.
Auteure/Auteur
David, J.P.
Auteure/Auteur
Guittard, E.
Auteure/Auteur
Maria, A.
Auteure/Auteur
Jacques, J.C.
Auteure/Auteur
Ranson, H.
Auteure/Auteur
Bourgouin, C.
Auteure/Auteur
Dauphin-Villemant, C.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1932-6203
Statut éditorial
Publié
Date de publication
2013
Volume
8
Numéro
12
Première page
e79861
Langue
anglais
Résumé
In insects, the steroid hormone 20-hydroxyecdysone (20E) coordinates major developmental transitions. While the first and the final steps of 20E biosynthesis are characterized, the pathway from 7-dehydrocholesterol to 5β-ketodiol, commonly referred as the "black box", remains hypothetical and whether there are still unidentified enzymes is unknown. The black box would include some oxidative steps, which are believed to be mediated by P450 enzymes. To identify new enzyme(s) involved in steroid synthesis, we analyzed by small-scale microarray the expression of all the genes encoding P450 enzymes of the malaria mosquito Anopheles gambiae in active steroidogenic organs of adults, ovaries from blood-fed females and male reproductive tracts, compared to inactive steroidogenic organs, ovaries from non-blood-fed females. Some genes encoding P450 enzymes were specifically overexpressed in female ovaries after a blood-meal or in male reproductive tracts but only three genes were found to be overexpressed in active steroidogenic organs of both females and males: cyp307a1, cyp4g16 and cyp6n1. Among these genes, only cyp307a1 has an expression pattern similar to other mosquito steroidogenic genes. Moreover, loss-of-function by transient RNAi targeting cyp307a1 disrupted ecdysteroid production demonstrating that this gene is required for ecdysteroid biosynthesis in Anopheles gambiae.
PID Serval
serval:BIB_6811F2BBCF63
PMID
Open Access
Oui
Date de création
2014-01-06T09:20:17.944Z
Date de création dans IRIS
2025-05-21T02:31:26Z
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Nom
BIB_6811F2BBCF63.P001.pdf
Version du manuscrit
preprint
Taille
610.47 KB
Format
Adobe PDF
PID Serval
serval:BIB_6811F2BBCF63.P001
URN
urn:nbn:ch:serval-BIB_6811F2BBCF638
Somme de contrôle
(MD5):f4a320c1dbcafd6064440bb246a7f876