Titre
MRI of iron-oxide labelled transplanted hepatocytes in mice: effect of treatment with cyclophosphamide.
Type
article
Institution
Externe
Périodique
Auteur(s)
Leconte, I.
Auteure/Auteur
Pallu, S.
Auteure/Auteur
Abarca-Quinones, J.
Auteure/Auteur
Michoux, N.
Auteure/Auteur
Peeters, F.
Auteure/Auteur
Radermacher, K.
Auteure/Auteur
Sempoux, C.
Auteure/Auteur
Najimi, M.
Auteure/Auteur
Sokal, E.
Auteure/Auteur
Van Beers, B.E.
Auteure/Auteur
Liens vers les personnes
ISSN
1522-2586
Statut éditorial
Publié
Date de publication
2010
Volume
32
Numéro
2
Première page
367
Dernière page/numéro d’article
375
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article Publication Status: ppublish
Résumé
PURPOSE: To assess if 1.5T MRI can be used to study the transport to the liver, the intrahepatic distribution and engraftment of iron-oxide labelled hepatocytes in cyclophosphamide-treated and untreated mice.
MATERIALS AND METHODS: Hepatocytes were isolated from C57bl/6 mice and were labelled with 1.63 microm iron-oxide particles. Seventeen mice were pretreated with cyclophosphamide to disrupt the sinusoidal endothelium and 15 were left untreated. Seven days after splenic injection of the labelled hepatocytes, T2*-weighted gradient-echo images at 1.5T were acquired. The hepatic transport, distribution and engraftment of the labelled hepatocytes were assessed with signal intensity (SI) and T2* measurements, electron paramagnetic resonance (EPR), texture analysis and histology.
RESULTS: Lower hepatic SI (P = 0.005), lower T2* (P = 0.033) and larger number of particles at histology (P = 0.006) suggested increased transport to the liver of labelled hepatocytes in cyclophosphamide-treated mice versus untreated mice. At histology, most particles were located in Kupffer cells. Particles distribution was heterogeneous. No difference between both groups was observed at texture analysis.
CONCLUSION: MRI is useful to assess the transport to the liver and intrahepatic distribution of transplanted labelled hepatocytes. The preferential location of iron-oxide particles within Kupffer cells after seven days limits the value of MRI for assessing hepatocyte engraftment.
MATERIALS AND METHODS: Hepatocytes were isolated from C57bl/6 mice and were labelled with 1.63 microm iron-oxide particles. Seventeen mice were pretreated with cyclophosphamide to disrupt the sinusoidal endothelium and 15 were left untreated. Seven days after splenic injection of the labelled hepatocytes, T2*-weighted gradient-echo images at 1.5T were acquired. The hepatic transport, distribution and engraftment of the labelled hepatocytes were assessed with signal intensity (SI) and T2* measurements, electron paramagnetic resonance (EPR), texture analysis and histology.
RESULTS: Lower hepatic SI (P = 0.005), lower T2* (P = 0.033) and larger number of particles at histology (P = 0.006) suggested increased transport to the liver of labelled hepatocytes in cyclophosphamide-treated mice versus untreated mice. At histology, most particles were located in Kupffer cells. Particles distribution was heterogeneous. No difference between both groups was observed at texture analysis.
CONCLUSION: MRI is useful to assess the transport to the liver and intrahepatic distribution of transplanted labelled hepatocytes. The preferential location of iron-oxide particles within Kupffer cells after seven days limits the value of MRI for assessing hepatocyte engraftment.
PID Serval
serval:BIB_437CB3207535
PMID
Date de création
2015-01-29T11:41:54.393Z
Date de création dans IRIS
2025-05-20T15:04:15Z