Titre
Release of N-acetylaspartylglutamate from slices of rat cerebellum, striatum, and spinal cord, and the effect of climbing fiber deprivation.
Type
article
Institution
Externe
Périodique
Auteur(s)
Zollinger, M.
Auteure/Auteur
Brauchli-Theotokis, J.
Auteure/Auteur
Gutteck-Amsler, U.
Auteure/Auteur
Do, K.Q.
Auteure/Auteur
Streit, P.
Auteure/Auteur
Cuénod, M.
Auteure/Auteur
Liens vers les personnes
ISSN
0022-3042
Statut éditorial
Publié
Date de publication
1994
Volume
63
Numéro
3
Première page
1133
Dernière page/numéro d’article
1142
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: In Vitro ; Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
The release of endogenous N-acetylaspartylglutamate (NAAG) from slices of rat cerebellum, striatum, and spinal cord upon depolarization with 50 mM K+ was investigated. NAAG in superfusates was prepurified using an ion exchanger, esterified, and then quantified by gas chromatography-mass spectrometry. Deuterated NAAG was used as internal standard. A depolarization-induced release of NAAG was found in all three regions. The release was Ca2+ dependent to over 85% in cerebellum and striatum, but only to approximately 70% in spinal cord. In addition, the effect of lesions of the olivocerebellar pathway on the K(+)-induced release of NAAG was studied: Treatment of the animals with 3-acetylpyridine reduced the release of NAAG from cerebellar hemispheres significantly, by about 40% compared with controls. These results suggest that part of the NAAG released from cerebellar slices on depolarization is related to climbing fibers. Implications of these findings concerning possible physiological roles of NAAG in the three CNS regions are discussed.
Sujets
PID Serval
serval:BIB_839C79FC167D
PMID
Date de création
2014-03-07T07:55:26.781Z
Date de création dans IRIS
2025-05-21T04:17:09Z