Titre
Glucose transporter 2 mediates the hypoglycemia-induced increase in cerebral blood flow.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Lei, H.
Auteure/Auteur
Preitner, F.
Auteure/Auteur
Labouèbe, G.
Auteure/Auteur
Gruetter, R.
Auteure/Auteur
Thorens, B.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1559-7016
Statut éditorial
Publié
Date de publication
2019-09
Volume
39
Numéro
9
Première page
1725
Dernière page/numéro d’article
1736
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Résumé
Glucose transporter 2 (Glut2)-positive cells are sparsely distributed in brain and play an important role in the stimulation of glucagon secretion in response to hypoglycemia. We aimed to determine if Glut2-positive cells can influence another response to hypoglycemia, i.e. increased cerebral blood flow (CBF). CBF of adult male mice devoid of Glut2, either globally (ripglut1:glut2 <sup>-</sup> <sup>/</sup> <sup>-</sup> ) or in the nervous system only (NG2KO), and their respective controls were studied under basal glycemia and insulin-induced hypoglycemia using quantitative perfusion magnetic resonance imaging at 9.4 T. The effect on CBF of optogenetic activation of hypoglycemia responsive Glut2-positive neurons of the paraventricular thalamic area was measured in mice expressing channelrhodopsin2 under the control of the Glut2 promoter. We found that in both ripglut1:glut2 <sup>-</sup> <sup>/</sup> <sup>-</sup> mice and NG2KO mice, CBF in basal conditions was higher than in their respective controls and not further activated by hypoglycemia, as measured in the hippocampus, hypothalamus and whole brain. Conversely, optogenetic activation of Glut2-positive cells in the paraventricular thalamic nucleus induced a local increase in CBF similar to that induced by hypoglycemia. Thus, Glut2 expression in the nervous system is required for the control of CBF in response to changes in blood glucose concentrations.
PID Serval
serval:BIB_FAB7A3D6BC78
PMID
Open Access
Oui
Date de création
2018-03-22T16:36:02.140Z
Date de création dans IRIS
2025-05-21T06:14:19Z
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Nom
JCBFM Labouèbe et al..pdf
Version du manuscrit
postprint
Taille
8.09 MB
Format
Adobe PDF
PID Serval
serval:BIB_FAB7A3D6BC78.P001
URN
urn:nbn:ch:serval-BIB_FAB7A3D6BC784
Somme de contrôle
(MD5):499345dd200dbf8950beac4734833501