Titre
Amplification and overexpression of PRUNE in human sarcomas and breast carcinomas-a possible mechanism for altering the nm23-H1 activity.
Type
article
Institution
Externe
Périodique
Auteur(s)
Forus, A.
Auteure/Auteur
D'Angelo, A.
Auteure/Auteur
Henriksen, J.
Auteure/Auteur
Merla, G.
Auteure/Auteur
Maelandsmo, G.M.
Auteure/Auteur
Flørenes, V.A.
Auteure/Auteur
Olivieri, S.
Auteure/Auteur
Bjerkehagen, B.
Auteure/Auteur
Meza-Zepeda, L.A.
Auteure/Auteur
del Vecchio Blanco, F.
Auteure/Auteur
Müller, C.
Auteure/Auteur
Sanvito, F.
Auteure/Auteur
Kononen, J.
Auteure/Auteur
Nesland, J.M.
Auteure/Auteur
Fodstad, Ø.
Auteure/Auteur
Reymond, A.
Auteure/Auteur
Kallioniemi, O.P.
Auteure/Auteur
Arrigoni, G.
Auteure/Auteur
Ballabio, A.
Auteure/Auteur
Myklebost, O.
Auteure/Auteur
Zollo, M.
Auteure/Auteur
Liens vers les personnes
ISSN
0950-9232[print], 0950-9232[linking]
Statut éditorial
Publié
Date de publication
2001
Volume
20
Numéro
47
Première page
6881
Dernière page/numéro d’article
6890
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Résumé
PRUNE, the human homologue of the Drosophila gene, is located in 1q21.3, a region highly amplified in human sarcomas, malignant tumours of mesenchymal origin. Prune protein interacts with the metastasis suppressor nm23-H1, but shows impaired affinity towards the nm23-H1 S120G mutant associated with advanced neuroblastoma. Based on these observations, we previously suggested that prune may act as a negative regulator of nm23-H1 activity. We found amplification of PRUNE in aggressive sarcoma subtypes, such as leiomyosarcomas and malignant fibrous histiocytomas (MFH) as well as in the less malignant liposarcomas. PRUNE amplification was generally accompanied by high mRNA and moderate to high protein levels. The sarcoma samples expressed nm23-H1 mostly at low or moderate levels, whereas mRNA and protein levels were moderate to high in breast carcinomas. For the more aggressive sarcoma subtypes, 9/13 patients with PRUNE amplification developed metastases. A similar situation was observed in all breast carcinomas with amplification of PRUNE. Infection of NIH3T3 cells with a PRUNE recombinant retrovirus increased cell proliferation. Possibly, amplification and overexpression of PRUNE has the same effect in the tumours. We suggest that amplification and overexpression of PRUNE could be a mechanism for inhibition of nm23-H1 activity that affect the development or progression of these tumours.
Sujets
PID Serval
serval:BIB_D4E69DCF698F
PMID
Open Access
Oui
Date de création
2009-05-11T10:49:47.455Z
Date de création dans IRIS
2025-05-20T21:53:24Z