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  4. Detection of Trichophyton rubrum and Trichophyton interdigitale in onychomycosis using monoclonal antibodies against Sub6 (Tri r 2).
 
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Titre

Detection of Trichophyton rubrum and Trichophyton interdigitale in onychomycosis using monoclonal antibodies against Sub6 (Tri r 2).

Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Mycoses: Diagnosis, Therapy and Prophylaxis of Fungal Diseases  
Auteur(s)
Méhul, B.
Auteure/Auteur
de Coi, N.
Auteure/Auteur
Grundt, P.
Auteure/Auteur
Genette, A.
Auteure/Auteur
Voegel, J.J.
Auteure/Auteur
Monod, M.
Auteure/Auteur
Liens vers les personnes
De Coi, Nicolo  
Monod, Michel  
Liens vers les unités
Dermatologie  
ISSN
1439-0507
Statut éditorial
Publié
Date de publication
2019-01
Volume
62
Numéro
1
Première page
32
Dernière page/numéro d’article
40
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Onychomycosis is the most prevalent nail disease and is mainly caused by two dermatophyte species Trichophyton rubrum and Trichophyton interdigitale with a frequency in the range of 80% and 20%, respectively. The secreted protease Sub6 of the subtilisin family, which was never detected in vitro growth conditions, was found to be a robust marker of onychomycosis.
The aim of this work was to detect tinea unguium using anti-Sub6 monoclonal antibodies in proteins extracted from clinical nail samples.
We produced monoclonal antibodies in mice using recombinant Sub6 as an antigen. Selected monoclonal antibodies were tested by Western blot analysis and ELISA on protein extracts from onychomycosis samples.
Several monoclonal antibodies used to quantify Sub6 in proteins extracted from clinical nail samples were produced and characterised. We showed that these antibodies were very specific and allowed the detection of T. rubrum and T. interdigitale in onychomycosis. Sub6 was detected in clinical samples infected by T. rubrum and not detected in nails with trauma and other diseases.
Anti-Sub6 monoclonal antibodies could be useful for a rapid diagnosis of tinea unguium and/or therapeutic survey of dermatophyte in onychomycosis by ELISA or an immunochromatography device such as a strip test.
Sujets

Animals

Antibodies, Fungal/im...

Antibodies, Monoclona...

Antigens, Fungal/anal...

Blotting, Western/met...

Enzyme-Linked Immunos...

Humans

Immunoassay/methods

Mice

Onychomycosis/diagnos...

Onychomycosis/microbi...

Peptide Hydrolases/an...

Sensitivity and Speci...

Trichophyton/isolatio...

Trichophyton interdig...

Trichophyton rubrum

Onychomycosis

Sub6

Tri r 2

monoclonal antibody

PID Serval
serval:BIB_BF7E38C6B860
DOI
10.1111/myc.12843
PMID
30152879
WOS
000453770400005
Permalien
https://iris.unil.ch/handle/iris/160567
Open Access
Oui
Date de création
2018-09-03T12:12:47.341Z
Date de création dans IRIS
2025-05-20T23:19:35Z
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