Titre
Microscopy of Fission Yeast Sexual Lifecycle.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Vjestica, A.
Auteure/Auteur
Merlini, L.
Auteure/Auteur
Dudin, O.
Auteure/Auteur
Bendezu, F.O.
Auteure/Auteur
Martin, S.G.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1940-087X
Statut éditorial
Publié
Date de publication
2016
Numéro
109
Première page
e53801
Langue
anglais
Résumé
The fission yeast Schizosaccharomyces pombe has been an invaluable model system in studying the regulation of the mitotic cell cycle progression, the mechanics of cell division and cell polarity. Furthermore, classical experiments on its sexual reproduction have yielded results pivotal to current understanding of DNA recombination and meiosis. More recent analysis of fission yeast mating has raised interesting questions on extrinsic stimuli response mechanisms, polarized cell growth and cell-cell fusion. To study these topics in detail we have developed a simple protocol for microscopy of the entire sexual lifecycle. The method described here is easily adjusted to study specific mating stages. Briefly, after being grown to exponential phase in a nitrogen-rich medium, cell cultures are shifted to a nitrogen-deprived medium for periods of time suited to the stage of the sexual lifecycle that will be explored. Cells are then mounted on custom, easily built agarose pad chambers for imaging. This approach allows cells to be monitored from the onset of mating to the final formation of spores.
PID Serval
serval:BIB_1DE423310FC6
PMID
Date de création
2016-04-09T14:09:34.497Z
Date de création dans IRIS
2025-05-20T17:22:25Z