Titre
Preferential infection of immature dendritic cells and B cells by mouse mammary tumor virus.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Vacheron, S.
Auteure/Auteur
Luther, S.A.
Auteure/Auteur
Acha-Orbea, H.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0022-1767[print], 0022-1767[linking]
Statut éditorial
Publié
Date de publication
2002
Volume
168
Numéro
7
Première page
3470
Dernière page/numéro d’article
3476
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Résumé
Until now it was thought that the retrovirus mouse mammary tumor virus preferentially infects B cells, which thereafter proliferate and differentiate due to superantigen-mediated T cell help. We describe in this study that dendritic cells are infectable at levels comparable to B cells in the first days after virus injection. Moreover, IgM knockout mice have chronically deleted superantigen-reactive T cells after MMTV injection, indicating that superantigen presentation by dendritic cells is sufficient for T cell deletion. In both subsets initially only few cells were infected, but there was an exponential increase in numbers of infected B cells due to superantigen-mediated T cell help, explaining that at the peak of the response infection is almost exclusively found in B cells. The level of infection in vivo was below 1 in 1000 dendritic cells or B cells. Infection levels in freshly isolated dendritic cells from spleen, Langerhans cells from skin, or bone marrow-derived dendritic cells were compared in an in vitro infection assay. Immature dendritic cells such as Langerhans cells or bone marrow-derived dendritic cells were infected 10- to 30-fold more efficiently than mature splenic dendritic cells. Bone marrow-derived dendritic cells carrying an endogenous mouse mammary tumor virus superantigen were highly efficient at inducing a superantigen response in vivo. These results highlight the importance of professional APC and efficient T cell priming for the establishment of a persistent infection by mouse mammary tumor virus.
Sujets
PID Serval
serval:BIB_E08993B57D6F
PMID
Date de création
2008-01-24T13:48:25.402Z
Date de création dans IRIS
2025-05-21T05:13:50Z