Titre
Identification of active oxalotrophic bacteria by Bromodeoxyuridine DNA labeling in a microcosm soil experiments
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Bravo, D.
Auteure/Auteur
Martin, G.
Auteure/Auteur
David, M.M.
Auteure/Auteur
Cailleau, G.
Auteure/Auteur
Verrecchia, E.
Auteure/Auteur
Junier, P.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0378-1097
Statut éditorial
Publié
Date de publication
2013
Volume
348
Première page
103
Dernière page/numéro d’article
111
Langue
anglais
Notes
ISI:000326056800003
Résumé
The oxalate-carbonate pathway (OCP) leads to a potential carbon sink in
terrestrial environments. This process is linked to the activity of
oxalotrophic bacteria. Although isolation and molecular
characterizations are used to study oxalotrophic bacteria, these
approaches do not give information on the active oxalotrophs present in
soil undergoing the OCP. The aim of this study was to assess the
diversity of active oxalotrophic bacteria in soil microcosms using the
Bromodeoxyuridine (BrdU) DNA labeling technique. Soil was collected near
an oxalogenic tree (Milicia excelsa). Different concentrations of
calcium oxalate (0.5%, 1%, and 4% w/w) were added to the soil
microcosms and compared with an untreated control. After 12days of
incubation, a maximal pH of 7.7 was measured for microcosms with oxalate
(initial pH 6.4). At this time point, a DGGE profile of the frc gene was
performed from BrdU-labeled soil DNA and unlabeled soil DNA.
Actinobacteria (Streptomyces- and Kribbella-like sequences),
Gammaproteobacteria and Betaproteobacteria were found as the main active
oxalotrophic bacterial groups. This study highlights the relevance of
Actinobacteria as members of the active bacterial community and the
identification of novel uncultured oxalotrophic groups (i.e. Kribbella)
active in soils.
terrestrial environments. This process is linked to the activity of
oxalotrophic bacteria. Although isolation and molecular
characterizations are used to study oxalotrophic bacteria, these
approaches do not give information on the active oxalotrophs present in
soil undergoing the OCP. The aim of this study was to assess the
diversity of active oxalotrophic bacteria in soil microcosms using the
Bromodeoxyuridine (BrdU) DNA labeling technique. Soil was collected near
an oxalogenic tree (Milicia excelsa). Different concentrations of
calcium oxalate (0.5%, 1%, and 4% w/w) were added to the soil
microcosms and compared with an untreated control. After 12days of
incubation, a maximal pH of 7.7 was measured for microcosms with oxalate
(initial pH 6.4). At this time point, a DGGE profile of the frc gene was
performed from BrdU-labeled soil DNA and unlabeled soil DNA.
Actinobacteria (Streptomyces- and Kribbella-like sequences),
Gammaproteobacteria and Betaproteobacteria were found as the main active
oxalotrophic bacterial groups. This study highlights the relevance of
Actinobacteria as members of the active bacterial community and the
identification of novel uncultured oxalotrophic groups (i.e. Kribbella)
active in soils.
PID Serval
serval:BIB_76E2485DE669
URL éditeur
Open Access
Oui
Date de création
2014-02-17T11:47:02.057Z
Date de création dans IRIS
2025-05-20T23:26:13Z
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REF.pdf
Version du manuscrit
published
Taille
244.07 KB
Format
Adobe PDF
PID Serval
serval:BIB_76E2485DE669.P001
URN
urn:nbn:ch:serval-BIB_76E2485DE6696
Somme de contrôle
(MD5):34cbf52afdb65f602854019486655c07