Titre
YscU recognizes translocators as export substrates of the Yersinia injectisome.
Type
article
Institution
Externe
Périodique
Auteur(s)
Sorg, I.
Auteure/Auteur
Wagner, S.
Auteure/Auteur
Amstutz, M.
Auteure/Auteur
Müller, S.A.
Auteure/Auteur
Broz, P.
Auteure/Auteur
Lussi, Y.
Auteure/Auteur
Engel, A.
Auteure/Auteur
Cornelis, G.R.
Auteure/Auteur
Liens vers les personnes
ISSN
0261-4189
Statut éditorial
Publié
Date de publication
2007
Volume
26
Numéro
12
Première page
3015
Dernière page/numéro d’article
3024
Peer-reviewed
Oui
Langue
anglais
Résumé
YscU is an essential component of the export apparatus of the Yersinia injectisome. It consists of an N-terminal transmembrane domain and a long cytoplasmic C-terminal domain, which undergoes auto-cleavage at a NPTH site. Substitutions N263A and P264A prevented cleavage of YscU and abolished export of LcrV, YopB and YopD but not of Yop effectors. As a consequence, yscU(N263A) mutant bacteria made needles without the LcrV tip complex and they could not form translocation pores. The graft of the export signal of the effector YopE, at the N-terminus of LcrV, restored LcrV export and assembly of the tip complex. Thus, YscU cleavage is required to acquire the conformation allowing recognition of translocators, which represent an individual category of substrates in the hierarchy of export. In addition, yscU(N263A) mutant bacteria exported reduced amounts of the YscP ruler and made longer needles. Increasing YscP export resulted in needles with normal size, depending on the length of the ruler. Hence, the effect of the yscU(N263A) mutation on needle length was the consequence of a reduced YscP export.
PID Serval
serval:BIB_BB4308686C43
PMID
Open Access
Oui
Date de création
2017-10-25T09:05:22.842Z
Date de création dans IRIS
2025-05-21T02:21:25Z